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Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways
Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict cl...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607220/ https://www.ncbi.nlm.nih.gov/pubmed/23041313 http://dx.doi.org/10.1016/j.celrep.2012.08.029 |
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author | Morris, Samantha A. Guo, Yu Zernicka-Goetz, Magdalena |
author_facet | Morris, Samantha A. Guo, Yu Zernicka-Goetz, Magdalena |
author_sort | Morris, Samantha A. |
collection | PubMed |
description | Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict clock of developmental transitions as intact embryos, but their potential is not equal. We have determined that unless a minimum of four pluripotent cells is established before implantation, development will arrest. This failure can be rescued by modulating Fgf and Wnt signaling to enhance pluripotent cell number, allowing the generation of monozygotic twins, which is an otherwise rare phenomenon. Knowledge of the minimum pluripotent-cell number required for development to birth, as well as the different potentials of blastomeres, allowed us to establish a protocol for splitting an embryo into one part that develops to adulthood and another that provides embryonic stem cells for that individual. |
format | Online Article Text |
id | pubmed-3607220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36072202013-03-25 Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways Morris, Samantha A. Guo, Yu Zernicka-Goetz, Magdalena Cell Rep Report Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict clock of developmental transitions as intact embryos, but their potential is not equal. We have determined that unless a minimum of four pluripotent cells is established before implantation, development will arrest. This failure can be rescued by modulating Fgf and Wnt signaling to enhance pluripotent cell number, allowing the generation of monozygotic twins, which is an otherwise rare phenomenon. Knowledge of the minimum pluripotent-cell number required for development to birth, as well as the different potentials of blastomeres, allowed us to establish a protocol for splitting an embryo into one part that develops to adulthood and another that provides embryonic stem cells for that individual. Cell Press 2012-10-25 /pmc/articles/PMC3607220/ /pubmed/23041313 http://dx.doi.org/10.1016/j.celrep.2012.08.029 Text en © 2012 The Authors https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Report Morris, Samantha A. Guo, Yu Zernicka-Goetz, Magdalena Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title_full | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title_fullStr | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title_full_unstemmed | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title_short | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
title_sort | developmental plasticity is bound by pluripotency and the fgf and wnt signaling pathways |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607220/ https://www.ncbi.nlm.nih.gov/pubmed/23041313 http://dx.doi.org/10.1016/j.celrep.2012.08.029 |
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