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Activation Energy of Extracellular Enzymes in Soils from Different Biomes

Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (E(a)) in soils and fewer studies have measured E(a) in a...

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Autores principales: Steinweg, J. Megan, Jagadamma, Sindhu, Frerichs, Joshua, Mayes, Melanie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607567/
https://www.ncbi.nlm.nih.gov/pubmed/23536898
http://dx.doi.org/10.1371/journal.pone.0059943
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author Steinweg, J. Megan
Jagadamma, Sindhu
Frerichs, Joshua
Mayes, Melanie A.
author_facet Steinweg, J. Megan
Jagadamma, Sindhu
Frerichs, Joshua
Mayes, Melanie A.
author_sort Steinweg, J. Megan
collection PubMed
description Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (E(a)) in soils and fewer studies have measured E(a) in arctic and tropical soils, or in subsurface soils. We determined the E(a) for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in E(a). β-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60°C. E(a) was calculated using the Arrhenius equation. β-glucosidase and cellobiohydrolase E(a) values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme E(a) and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between E(a) and pH in both horizons. The E(a) in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme E(a) values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones.
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spelling pubmed-36075672013-03-27 Activation Energy of Extracellular Enzymes in Soils from Different Biomes Steinweg, J. Megan Jagadamma, Sindhu Frerichs, Joshua Mayes, Melanie A. PLoS One Research Article Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (E(a)) in soils and fewer studies have measured E(a) in arctic and tropical soils, or in subsurface soils. We determined the E(a) for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in E(a). β-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60°C. E(a) was calculated using the Arrhenius equation. β-glucosidase and cellobiohydrolase E(a) values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme E(a) and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between E(a) and pH in both horizons. The E(a) in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme E(a) values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones. Public Library of Science 2013-03-25 /pmc/articles/PMC3607567/ /pubmed/23536898 http://dx.doi.org/10.1371/journal.pone.0059943 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Steinweg, J. Megan
Jagadamma, Sindhu
Frerichs, Joshua
Mayes, Melanie A.
Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title_full Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title_fullStr Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title_full_unstemmed Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title_short Activation Energy of Extracellular Enzymes in Soils from Different Biomes
title_sort activation energy of extracellular enzymes in soils from different biomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607567/
https://www.ncbi.nlm.nih.gov/pubmed/23536898
http://dx.doi.org/10.1371/journal.pone.0059943
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