Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples

Most Escherichia coli resistant to quinolones are not haemolytic. The objective of this study was to determine the phylogroup, clonal relationship, mechanism of quinolone resistance and virulence factors in 70 haemolytic E. coli resistant to nalidixic acid. Sixty-six isolates contained the hlyA gene...

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Autores principales: Márquez-López, Alicia, Ruiz del Castillo, Belén, Cano, María Eliecer, Rodríguez-Mirones, Cristina, Oteo, Jesús, Sáez, David, Martínez-Martínez, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607716/
https://www.ncbi.nlm.nih.gov/pubmed/23543875
http://dx.doi.org/10.1186/2193-1801-2-71
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author Márquez-López, Alicia
Ruiz del Castillo, Belén
Cano, María Eliecer
Rodríguez-Mirones, Cristina
Oteo, Jesús
Sáez, David
Martínez-Martínez, Luis
author_facet Márquez-López, Alicia
Ruiz del Castillo, Belén
Cano, María Eliecer
Rodríguez-Mirones, Cristina
Oteo, Jesús
Sáez, David
Martínez-Martínez, Luis
author_sort Márquez-López, Alicia
collection PubMed
description Most Escherichia coli resistant to quinolones are not haemolytic. The objective of this study was to determine the phylogroup, clonal relationship, mechanism of quinolone resistance and virulence factors in 70 haemolytic E. coli resistant to nalidixic acid. Sixty-six isolates contained the hlyA gene, belonged to phylogroup B2, and 61 of them presented low-level resistance to fluoroquinolones. Four isolates presented high-level resistance to fluoroquinolones, contained the clyA gene and were included in phylogroup D. One single isolate (phylogroup D, with low level resistance to fluoroquinolones) contained both cytotoxins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-71) contains supplementary material, which is available to authorized users.
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spelling pubmed-36077162013-03-27 Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples Márquez-López, Alicia Ruiz del Castillo, Belén Cano, María Eliecer Rodríguez-Mirones, Cristina Oteo, Jesús Sáez, David Martínez-Martínez, Luis Springerplus Research Most Escherichia coli resistant to quinolones are not haemolytic. The objective of this study was to determine the phylogroup, clonal relationship, mechanism of quinolone resistance and virulence factors in 70 haemolytic E. coli resistant to nalidixic acid. Sixty-six isolates contained the hlyA gene, belonged to phylogroup B2, and 61 of them presented low-level resistance to fluoroquinolones. Four isolates presented high-level resistance to fluoroquinolones, contained the clyA gene and were included in phylogroup D. One single isolate (phylogroup D, with low level resistance to fluoroquinolones) contained both cytotoxins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-71) contains supplementary material, which is available to authorized users. Springer International Publishing AG 2013-02-27 /pmc/articles/PMC3607716/ /pubmed/23543875 http://dx.doi.org/10.1186/2193-1801-2-71 Text en © Márquez-López et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Márquez-López, Alicia
Ruiz del Castillo, Belén
Cano, María Eliecer
Rodríguez-Mirones, Cristina
Oteo, Jesús
Sáez, David
Martínez-Martínez, Luis
Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title_full Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title_fullStr Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title_full_unstemmed Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title_short Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples
title_sort production of hlya and clya haemolysins among quinolone-resistant escherichia coli isolated from clinical samples
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607716/
https://www.ncbi.nlm.nih.gov/pubmed/23543875
http://dx.doi.org/10.1186/2193-1801-2-71
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