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Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality

Tuber yield, starch content, starch yield and chip color are complex traits that are important for industrial uses and food processing of potato. Chip color depends on the quantity of reducing sugars glucose and fructose in the tubers, which are generated by starch degradation. Reducing sugars accum...

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Autores principales: Li, Li, Tacke, Eckhard, Hofferbert, Hans-Reinhardt, Lübeck, Jens, Strahwald, Josef, Draffehn, Astrid M., Walkemeier, Birgit, Gebhardt, Christiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607734/
https://www.ncbi.nlm.nih.gov/pubmed/23299900
http://dx.doi.org/10.1007/s00122-012-2035-z
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author Li, Li
Tacke, Eckhard
Hofferbert, Hans-Reinhardt
Lübeck, Jens
Strahwald, Josef
Draffehn, Astrid M.
Walkemeier, Birgit
Gebhardt, Christiane
author_facet Li, Li
Tacke, Eckhard
Hofferbert, Hans-Reinhardt
Lübeck, Jens
Strahwald, Josef
Draffehn, Astrid M.
Walkemeier, Birgit
Gebhardt, Christiane
author_sort Li, Li
collection PubMed
description Tuber yield, starch content, starch yield and chip color are complex traits that are important for industrial uses and food processing of potato. Chip color depends on the quantity of reducing sugars glucose and fructose in the tubers, which are generated by starch degradation. Reducing sugars accumulate when tubers are stored at low temperatures. Early and efficient selection of cultivars with superior yield, starch yield and chip color is hampered by the fact that reliable phenotypic selection requires multiple year and location trials. Application of DNA-based markers early in the breeding cycle, which are diagnostic for superior alleles of genes that control natural variation of tuber quality, will reduce the number of clones to be evaluated in field trials. Association mapping using genes functional in carbohydrate metabolism as markers has discovered alleles of invertases and starch phosphorylases that are associated with tuber quality traits. Here, we report on new DNA variants at loci encoding ADP-glucose pyrophosphorylase and the invertase Pain-1, which are associated with positive or negative effect with chip color, tuber starch content and starch yield. Marker-assisted selection (MAS) and marker validation were performed in tetraploid breeding populations, using various combinations of 11 allele-specific markers associated with tuber quality traits. To facilitate MAS, user-friendly PCR assays were developed for specific candidate gene alleles. In a multi-parental population of advanced breeding clones, genotypes were selected for having different combinations of five positive and the corresponding negative marker alleles. Genotypes combining five positive marker alleles performed on average better than genotypes with four negative alleles and one positive allele. When tested individually, seven of eight markers showed an effect on at least one quality trait. The direction of effect was as expected. Combinations of two to three marker alleles were identified that significantly improved average chip quality after cold storage and tuber starch content. In F1 progeny of a single-cross combination, MAS with six markers did not give the expected result. Reasons and implications for MAS in potato are discussed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-012-2035-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-36077342013-03-27 Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality Li, Li Tacke, Eckhard Hofferbert, Hans-Reinhardt Lübeck, Jens Strahwald, Josef Draffehn, Astrid M. Walkemeier, Birgit Gebhardt, Christiane Theor Appl Genet Original Paper Tuber yield, starch content, starch yield and chip color are complex traits that are important for industrial uses and food processing of potato. Chip color depends on the quantity of reducing sugars glucose and fructose in the tubers, which are generated by starch degradation. Reducing sugars accumulate when tubers are stored at low temperatures. Early and efficient selection of cultivars with superior yield, starch yield and chip color is hampered by the fact that reliable phenotypic selection requires multiple year and location trials. Application of DNA-based markers early in the breeding cycle, which are diagnostic for superior alleles of genes that control natural variation of tuber quality, will reduce the number of clones to be evaluated in field trials. Association mapping using genes functional in carbohydrate metabolism as markers has discovered alleles of invertases and starch phosphorylases that are associated with tuber quality traits. Here, we report on new DNA variants at loci encoding ADP-glucose pyrophosphorylase and the invertase Pain-1, which are associated with positive or negative effect with chip color, tuber starch content and starch yield. Marker-assisted selection (MAS) and marker validation were performed in tetraploid breeding populations, using various combinations of 11 allele-specific markers associated with tuber quality traits. To facilitate MAS, user-friendly PCR assays were developed for specific candidate gene alleles. In a multi-parental population of advanced breeding clones, genotypes were selected for having different combinations of five positive and the corresponding negative marker alleles. Genotypes combining five positive marker alleles performed on average better than genotypes with four negative alleles and one positive allele. When tested individually, seven of eight markers showed an effect on at least one quality trait. The direction of effect was as expected. Combinations of two to three marker alleles were identified that significantly improved average chip quality after cold storage and tuber starch content. In F1 progeny of a single-cross combination, MAS with six markers did not give the expected result. Reasons and implications for MAS in potato are discussed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-012-2035-z) contains supplementary material, which is available to authorized users. Springer-Verlag 2013-01-09 2013 /pmc/articles/PMC3607734/ /pubmed/23299900 http://dx.doi.org/10.1007/s00122-012-2035-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Li, Li
Tacke, Eckhard
Hofferbert, Hans-Reinhardt
Lübeck, Jens
Strahwald, Josef
Draffehn, Astrid M.
Walkemeier, Birgit
Gebhardt, Christiane
Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title_full Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title_fullStr Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title_full_unstemmed Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title_short Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
title_sort validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607734/
https://www.ncbi.nlm.nih.gov/pubmed/23299900
http://dx.doi.org/10.1007/s00122-012-2035-z
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