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De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel
BACKGROUND: The yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathog...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608080/ https://www.ncbi.nlm.nih.gov/pubmed/23496978 http://dx.doi.org/10.1186/1471-2164-14-168 |
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author | Hershkovitz, Vera Sela, Noa Taha-Salaime, Leena Liu, Jia Rafael, Ginat Kessler, Clarita Aly, Radi Levy, Maggie Wisniewski, Michael Droby, Samir |
author_facet | Hershkovitz, Vera Sela, Noa Taha-Salaime, Leena Liu, Jia Rafael, Ginat Kessler, Clarita Aly, Radi Levy, Maggie Wisniewski, Michael Droby, Samir |
author_sort | Hershkovitz, Vera |
collection | PubMed |
description | BACKGROUND: The yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathogen, Penicillium digitatum. RESULTS: More than 26 million sequencing reads were assembled into 9,674 unigenes. Approximately 50% of the unigenes could be annotated based on homology matches in the NCBI database. Based on homology, sequences were annotated with a gene description, gene ontology (GO term), and clustered into functional groups. An analysis of differential expression when the yeast was interacting with the fruit vs. the pathogen revealed more than 250 genes with specific expression responses. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. Patterns of gene expression in the two interactions were examined at the individual transcript level by quantitative real-time PCR analysis (RT-qPCR). CONCLUSION: This study provides new insight into the biology of the tritrophic interactions that occur in a biocontrol system such as the use of the yeast, M. fructicola for the control of green mold on citrus caused by P. digitatum. |
format | Online Article Text |
id | pubmed-3608080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36080802013-03-27 De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel Hershkovitz, Vera Sela, Noa Taha-Salaime, Leena Liu, Jia Rafael, Ginat Kessler, Clarita Aly, Radi Levy, Maggie Wisniewski, Michael Droby, Samir BMC Genomics Research Article BACKGROUND: The yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathogen, Penicillium digitatum. RESULTS: More than 26 million sequencing reads were assembled into 9,674 unigenes. Approximately 50% of the unigenes could be annotated based on homology matches in the NCBI database. Based on homology, sequences were annotated with a gene description, gene ontology (GO term), and clustered into functional groups. An analysis of differential expression when the yeast was interacting with the fruit vs. the pathogen revealed more than 250 genes with specific expression responses. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. Patterns of gene expression in the two interactions were examined at the individual transcript level by quantitative real-time PCR analysis (RT-qPCR). CONCLUSION: This study provides new insight into the biology of the tritrophic interactions that occur in a biocontrol system such as the use of the yeast, M. fructicola for the control of green mold on citrus caused by P. digitatum. BioMed Central 2013-03-12 /pmc/articles/PMC3608080/ /pubmed/23496978 http://dx.doi.org/10.1186/1471-2164-14-168 Text en Copyright ©2013 Hershkovitz et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hershkovitz, Vera Sela, Noa Taha-Salaime, Leena Liu, Jia Rafael, Ginat Kessler, Clarita Aly, Radi Levy, Maggie Wisniewski, Michael Droby, Samir De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title | De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title_full | De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title_fullStr | De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title_full_unstemmed | De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title_short | De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel |
title_sort | de-novo assembly and characterization of the transcriptome of metschnikowia fructicola reveals differences in gene expression following interaction with penicillium digitatum and grapefruit peel |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608080/ https://www.ncbi.nlm.nih.gov/pubmed/23496978 http://dx.doi.org/10.1186/1471-2164-14-168 |
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