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Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects

BACKGROUND: The model of pulse plethysmograph using inhalational salbutamol 400 mcg is studied well to assess endothelium dependent vasodilation. Endothelial nitric oxide synthase (eNOS) gene polymorphism may influence the response to salbutamol in healthy subjects. AIM: To find the effect of polymo...

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Autores principales: Kumar, Srinivasamurthy Suresh, Kumar, Annan Sudarsan Arun, Padmapriya, Ramamoorthy, Chandrasekaran, Adithan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608305/
https://www.ncbi.nlm.nih.gov/pubmed/23543259
http://dx.doi.org/10.4103/0253-7613.106427
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author Kumar, Srinivasamurthy Suresh
Kumar, Annan Sudarsan Arun
Padmapriya, Ramamoorthy
Chandrasekaran, Adithan
author_facet Kumar, Srinivasamurthy Suresh
Kumar, Annan Sudarsan Arun
Padmapriya, Ramamoorthy
Chandrasekaran, Adithan
author_sort Kumar, Srinivasamurthy Suresh
collection PubMed
description BACKGROUND: The model of pulse plethysmograph using inhalational salbutamol 400 mcg is studied well to assess endothelium dependent vasodilation. Endothelial nitric oxide synthase (eNOS) gene polymorphism may influence the response to salbutamol in healthy subjects. AIM: To find the effect of polymorphisms 894G>T and -786T>C of eNOS gene on endothelium dependent vasodilation in healthy subjects. MATERIALS AND METHODS: One hundred and two south Indian healthy subjects of either sex, aged between 18 to 35 years were recruited for the study. The digital volume pulse (DVP)was measured by pulse plethysmograph before and after salbutamol 400mcg inhalation. Three predose and five postdose recordings of DVP were measured. The average change in the DVP parameters namely reflection index (RI) and stiffness index (SI) were determined. The eNOS894G>T and -786T>C gene polymorphism were genotyped using polymerase chain reaction followed by restriction fragment length polymorphism. The percentage changes in RI and SI from predose baseline recordings were calculated and compared between the genotype groups. RESULTS: The genotype and allele frequency of study subjects were in Hardy-Weinberg equilibrium. The changes in DVP parameters were not significantly different between the genotype groups. CONCLUSION: eNOS polymorphism do not affect salbutamol evoked endothelium dependent vasodilation in the model of pulse plethysmograph in healthy subjects.
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spelling pubmed-36083052013-03-29 Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects Kumar, Srinivasamurthy Suresh Kumar, Annan Sudarsan Arun Padmapriya, Ramamoorthy Chandrasekaran, Adithan Indian J Pharmacol Research Article BACKGROUND: The model of pulse plethysmograph using inhalational salbutamol 400 mcg is studied well to assess endothelium dependent vasodilation. Endothelial nitric oxide synthase (eNOS) gene polymorphism may influence the response to salbutamol in healthy subjects. AIM: To find the effect of polymorphisms 894G>T and -786T>C of eNOS gene on endothelium dependent vasodilation in healthy subjects. MATERIALS AND METHODS: One hundred and two south Indian healthy subjects of either sex, aged between 18 to 35 years were recruited for the study. The digital volume pulse (DVP)was measured by pulse plethysmograph before and after salbutamol 400mcg inhalation. Three predose and five postdose recordings of DVP were measured. The average change in the DVP parameters namely reflection index (RI) and stiffness index (SI) were determined. The eNOS894G>T and -786T>C gene polymorphism were genotyped using polymerase chain reaction followed by restriction fragment length polymorphism. The percentage changes in RI and SI from predose baseline recordings were calculated and compared between the genotype groups. RESULTS: The genotype and allele frequency of study subjects were in Hardy-Weinberg equilibrium. The changes in DVP parameters were not significantly different between the genotype groups. CONCLUSION: eNOS polymorphism do not affect salbutamol evoked endothelium dependent vasodilation in the model of pulse plethysmograph in healthy subjects. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3608305/ /pubmed/23543259 http://dx.doi.org/10.4103/0253-7613.106427 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kumar, Srinivasamurthy Suresh
Kumar, Annan Sudarsan Arun
Padmapriya, Ramamoorthy
Chandrasekaran, Adithan
Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title_full Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title_fullStr Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title_full_unstemmed Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title_short Effect of eNOS polymorphisms on salbutamol evoked endothelium dependent vasodilation in South Indian healthy subjects
title_sort effect of enos polymorphisms on salbutamol evoked endothelium dependent vasodilation in south indian healthy subjects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608305/
https://www.ncbi.nlm.nih.gov/pubmed/23543259
http://dx.doi.org/10.4103/0253-7613.106427
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