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Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile
BACKGROUND: Local IgE production may play a role in asthma pathogenesis. The aim of the study was to assess sputum total IgE and cytokines in asthmatics according to sputum cellular phenotype. METHODS: We studied 122 subjects including 22 non atopic healthy subjects, 41 eosinophilic (sputum eosinoph...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608646/ https://www.ncbi.nlm.nih.gov/pubmed/23555579 http://dx.doi.org/10.1371/journal.pone.0058388 |
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author | Manise, Maïté Holtappels, Gabriele Van Crombruggen, Koen Schleich, Florence Bachert, Claus Louis, Renaud |
author_facet | Manise, Maïté Holtappels, Gabriele Van Crombruggen, Koen Schleich, Florence Bachert, Claus Louis, Renaud |
author_sort | Manise, Maïté |
collection | PubMed |
description | BACKGROUND: Local IgE production may play a role in asthma pathogenesis. The aim of the study was to assess sputum total IgE and cytokines in asthmatics according to sputum cellular phenotype. METHODS: We studied 122 subjects including 22 non atopic healthy subjects, 41 eosinophilic (sputum eosinophils ≥3%), 16 neutrophilic (sputum neutrophils >76%) and 43 pauci-granulocytic asthmatics (sputum eosinophils <3% and sputum neutrophils ≤76%) recruited from the asthma clinic at CHU Liege. Sputum supernatant total IgE (tIgE) was measured by ImmunoCAP and sputum supernatant cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ and TNF-α) were measured with the Luminex xMAP Technology by using commercially available Fluorokine MAP kits. RESULTS: After concentrating sputum samples, total IgE was detectable in the majority of subjects. Sputum IgE was raised in asthmatics when compared to healthy subjects. Overall, asthmatics did not significantly differ from healthy subjects with respect to cytokine levels. The eosinophilic asthma phenotype, however, was characterised by raised sputum tIgE, IL-5 and IL-13 compared to healthy subjects (p<0.001, p<0.001 and p<0.05 respectively) and pauci-granulocytic asthma (p<0.01, p<0.001 and p<0.05 respectively) and raised IL-5 compared to neutrophilic asthma (p<0.01). When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-α sputum supernatant levels were raised in the “IgE high” asthmatics (IgE ≥0.1 kU/l) when compared to “IgE low” asthmatics (IgE<0.1 kU/l). CONCLUSION: The eosinophilic asthma phenotype was associated with raised sputum IgE and a Th2 cytokine profile. Raised sputum IgE was associated with a heterogeneous cytokine overproduction. |
format | Online Article Text |
id | pubmed-3608646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36086462013-04-03 Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile Manise, Maïté Holtappels, Gabriele Van Crombruggen, Koen Schleich, Florence Bachert, Claus Louis, Renaud PLoS One Research Article BACKGROUND: Local IgE production may play a role in asthma pathogenesis. The aim of the study was to assess sputum total IgE and cytokines in asthmatics according to sputum cellular phenotype. METHODS: We studied 122 subjects including 22 non atopic healthy subjects, 41 eosinophilic (sputum eosinophils ≥3%), 16 neutrophilic (sputum neutrophils >76%) and 43 pauci-granulocytic asthmatics (sputum eosinophils <3% and sputum neutrophils ≤76%) recruited from the asthma clinic at CHU Liege. Sputum supernatant total IgE (tIgE) was measured by ImmunoCAP and sputum supernatant cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ and TNF-α) were measured with the Luminex xMAP Technology by using commercially available Fluorokine MAP kits. RESULTS: After concentrating sputum samples, total IgE was detectable in the majority of subjects. Sputum IgE was raised in asthmatics when compared to healthy subjects. Overall, asthmatics did not significantly differ from healthy subjects with respect to cytokine levels. The eosinophilic asthma phenotype, however, was characterised by raised sputum tIgE, IL-5 and IL-13 compared to healthy subjects (p<0.001, p<0.001 and p<0.05 respectively) and pauci-granulocytic asthma (p<0.01, p<0.001 and p<0.05 respectively) and raised IL-5 compared to neutrophilic asthma (p<0.01). When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-α sputum supernatant levels were raised in the “IgE high” asthmatics (IgE ≥0.1 kU/l) when compared to “IgE low” asthmatics (IgE<0.1 kU/l). CONCLUSION: The eosinophilic asthma phenotype was associated with raised sputum IgE and a Th2 cytokine profile. Raised sputum IgE was associated with a heterogeneous cytokine overproduction. Public Library of Science 2013-03-26 /pmc/articles/PMC3608646/ /pubmed/23555579 http://dx.doi.org/10.1371/journal.pone.0058388 Text en © 2013 Manise et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Manise, Maïté Holtappels, Gabriele Van Crombruggen, Koen Schleich, Florence Bachert, Claus Louis, Renaud Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title | Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title_full | Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title_fullStr | Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title_full_unstemmed | Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title_short | Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile |
title_sort | sputum ige and cytokines in asthma: relationship with sputum cellular profile |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608646/ https://www.ncbi.nlm.nih.gov/pubmed/23555579 http://dx.doi.org/10.1371/journal.pone.0058388 |
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