Cargando…

RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)

Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Haiming, Yi, Xin, Hu, Zhen, Hu, Meiying, Chen, Shaohua, Muhammad, Rizwan-ul-Haq, Dong, Xiaolin, Gong, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608696/
https://www.ncbi.nlm.nih.gov/pubmed/23555693
http://dx.doi.org/10.1371/journal.pone.0059527
_version_ 1782264270894923776
author Zhao, Haiming
Yi, Xin
Hu, Zhen
Hu, Meiying
Chen, Shaohua
Muhammad, Rizwan-ul-Haq
Dong, Xiaolin
Gong, Liang
author_facet Zhao, Haiming
Yi, Xin
Hu, Zhen
Hu, Meiying
Chen, Shaohua
Muhammad, Rizwan-ul-Haq
Dong, Xiaolin
Gong, Liang
author_sort Zhao, Haiming
collection PubMed
description Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen, which decomposes harmful hydrogen peroxide, into water and oxygen. In this study, the full length sequence that encodes CAT-like protein from Spodoptera litura named siltCAT (GenBank accession number: JQ_663444) was cloned and characterized. Amino acid sequence alignment showed siltCAT shared relatively high conservation with other insect, especially the conserved residues which defined heme and NADPH orientation. Expression pattern analysis showed that siltCAT mRNA was mainly expressed in the fat body, midgut, cuticle and malpighian tube, and as well as over last instar larvae, pupa and adult stages. RNA interference was used to silence CAT gene in SL-1 cells and the fourth-instar stage of S. litura larvae respectively. Our results provided evidence that CAT knockdown induced ROS generation, cell cycle arrest and apoptosis in SL-1 cells. It also confirmed the decrease in survival rate because of increased ROS production in experimental groups injected with double-stranded RNA of CAT (dsCAT). This study implied that ROS scavenging by CAT is important for S. litura survival.
format Online
Article
Text
id pubmed-3608696
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36086962013-04-03 RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius) Zhao, Haiming Yi, Xin Hu, Zhen Hu, Meiying Chen, Shaohua Muhammad, Rizwan-ul-Haq Dong, Xiaolin Gong, Liang PLoS One Research Article Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen, which decomposes harmful hydrogen peroxide, into water and oxygen. In this study, the full length sequence that encodes CAT-like protein from Spodoptera litura named siltCAT (GenBank accession number: JQ_663444) was cloned and characterized. Amino acid sequence alignment showed siltCAT shared relatively high conservation with other insect, especially the conserved residues which defined heme and NADPH orientation. Expression pattern analysis showed that siltCAT mRNA was mainly expressed in the fat body, midgut, cuticle and malpighian tube, and as well as over last instar larvae, pupa and adult stages. RNA interference was used to silence CAT gene in SL-1 cells and the fourth-instar stage of S. litura larvae respectively. Our results provided evidence that CAT knockdown induced ROS generation, cell cycle arrest and apoptosis in SL-1 cells. It also confirmed the decrease in survival rate because of increased ROS production in experimental groups injected with double-stranded RNA of CAT (dsCAT). This study implied that ROS scavenging by CAT is important for S. litura survival. Public Library of Science 2013-03-26 /pmc/articles/PMC3608696/ /pubmed/23555693 http://dx.doi.org/10.1371/journal.pone.0059527 Text en © 2013 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhao, Haiming
Yi, Xin
Hu, Zhen
Hu, Meiying
Chen, Shaohua
Muhammad, Rizwan-ul-Haq
Dong, Xiaolin
Gong, Liang
RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title_full RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title_fullStr RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title_full_unstemmed RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title_short RNAi-Mediated Knockdown of Catalase Causes Cell Cycle Arrest in SL-1 Cells and Results in Low Survival Rate of Spodoptera litura (Fabricius)
title_sort rnai-mediated knockdown of catalase causes cell cycle arrest in sl-1 cells and results in low survival rate of spodoptera litura (fabricius)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608696/
https://www.ncbi.nlm.nih.gov/pubmed/23555693
http://dx.doi.org/10.1371/journal.pone.0059527
work_keys_str_mv AT zhaohaiming rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT yixin rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT huzhen rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT humeiying rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT chenshaohua rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT muhammadrizwanulhaq rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT dongxiaolin rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius
AT gongliang rnaimediatedknockdownofcatalasecausescellcyclearrestinsl1cellsandresultsinlowsurvivalrateofspodopteraliturafabricius