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The Influence of Electric Field and Confinement on Cell Motility

The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by...

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Detalles Bibliográficos
Autores principales: Huang, Yu-Ja, Samorajski, Justin, Kreimer, Rachel, Searson, Peter C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608730/
https://www.ncbi.nlm.nih.gov/pubmed/23555674
http://dx.doi.org/10.1371/journal.pone.0059447
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author Huang, Yu-Ja
Samorajski, Justin
Kreimer, Rachel
Searson, Peter C.
author_facet Huang, Yu-Ja
Samorajski, Justin
Kreimer, Rachel
Searson, Peter C.
author_sort Huang, Yu-Ja
collection PubMed
description The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by moving preferentially either parallel or antiparallel to the field vector, a process known as galvanotaxis. Here we report on the influence of dc electric field and confinement on the motility of fibroblast cells using a chip-based platform. From analysis of cell paths we show that the influence of electric field on motility is much more complex than simply imposing a directional bias towards the cathode or anode. The cell velocity, directedness, as well as the parallel and perpendicular components of the segments along the cell path are dependent on the magnitude of the electric field. Forces in the directions perpendicular and parallel to the electric field are in competition with one another in a voltage-dependent manner, which ultimately govern the trajectories of the cells in the presence of an electric field. To further investigate the effects of cell reorientation in the presence of a field, cells are confined within microchannels to physically prohibit the alignment seen in 2D environment. Interestingly, we found that confinement results in an increase in cell velocity both in the absence and presence of an electric field compared to migration in 2D.
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spelling pubmed-36087302013-04-03 The Influence of Electric Field and Confinement on Cell Motility Huang, Yu-Ja Samorajski, Justin Kreimer, Rachel Searson, Peter C. PLoS One Research Article The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by moving preferentially either parallel or antiparallel to the field vector, a process known as galvanotaxis. Here we report on the influence of dc electric field and confinement on the motility of fibroblast cells using a chip-based platform. From analysis of cell paths we show that the influence of electric field on motility is much more complex than simply imposing a directional bias towards the cathode or anode. The cell velocity, directedness, as well as the parallel and perpendicular components of the segments along the cell path are dependent on the magnitude of the electric field. Forces in the directions perpendicular and parallel to the electric field are in competition with one another in a voltage-dependent manner, which ultimately govern the trajectories of the cells in the presence of an electric field. To further investigate the effects of cell reorientation in the presence of a field, cells are confined within microchannels to physically prohibit the alignment seen in 2D environment. Interestingly, we found that confinement results in an increase in cell velocity both in the absence and presence of an electric field compared to migration in 2D. Public Library of Science 2013-03-26 /pmc/articles/PMC3608730/ /pubmed/23555674 http://dx.doi.org/10.1371/journal.pone.0059447 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Huang, Yu-Ja
Samorajski, Justin
Kreimer, Rachel
Searson, Peter C.
The Influence of Electric Field and Confinement on Cell Motility
title The Influence of Electric Field and Confinement on Cell Motility
title_full The Influence of Electric Field and Confinement on Cell Motility
title_fullStr The Influence of Electric Field and Confinement on Cell Motility
title_full_unstemmed The Influence of Electric Field and Confinement on Cell Motility
title_short The Influence of Electric Field and Confinement on Cell Motility
title_sort influence of electric field and confinement on cell motility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608730/
https://www.ncbi.nlm.nih.gov/pubmed/23555674
http://dx.doi.org/10.1371/journal.pone.0059447
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