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The Influence of Electric Field and Confinement on Cell Motility
The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608730/ https://www.ncbi.nlm.nih.gov/pubmed/23555674 http://dx.doi.org/10.1371/journal.pone.0059447 |
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author | Huang, Yu-Ja Samorajski, Justin Kreimer, Rachel Searson, Peter C. |
author_facet | Huang, Yu-Ja Samorajski, Justin Kreimer, Rachel Searson, Peter C. |
author_sort | Huang, Yu-Ja |
collection | PubMed |
description | The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by moving preferentially either parallel or antiparallel to the field vector, a process known as galvanotaxis. Here we report on the influence of dc electric field and confinement on the motility of fibroblast cells using a chip-based platform. From analysis of cell paths we show that the influence of electric field on motility is much more complex than simply imposing a directional bias towards the cathode or anode. The cell velocity, directedness, as well as the parallel and perpendicular components of the segments along the cell path are dependent on the magnitude of the electric field. Forces in the directions perpendicular and parallel to the electric field are in competition with one another in a voltage-dependent manner, which ultimately govern the trajectories of the cells in the presence of an electric field. To further investigate the effects of cell reorientation in the presence of a field, cells are confined within microchannels to physically prohibit the alignment seen in 2D environment. Interestingly, we found that confinement results in an increase in cell velocity both in the absence and presence of an electric field compared to migration in 2D. |
format | Online Article Text |
id | pubmed-3608730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36087302013-04-03 The Influence of Electric Field and Confinement on Cell Motility Huang, Yu-Ja Samorajski, Justin Kreimer, Rachel Searson, Peter C. PLoS One Research Article The ability of cells to sense and respond to endogenous electric fields is important in processes such as wound healing, development, and nerve regeneration. In cell culture, many epithelial and endothelial cell types respond to an electric field of magnitude similar to endogenous electric fields by moving preferentially either parallel or antiparallel to the field vector, a process known as galvanotaxis. Here we report on the influence of dc electric field and confinement on the motility of fibroblast cells using a chip-based platform. From analysis of cell paths we show that the influence of electric field on motility is much more complex than simply imposing a directional bias towards the cathode or anode. The cell velocity, directedness, as well as the parallel and perpendicular components of the segments along the cell path are dependent on the magnitude of the electric field. Forces in the directions perpendicular and parallel to the electric field are in competition with one another in a voltage-dependent manner, which ultimately govern the trajectories of the cells in the presence of an electric field. To further investigate the effects of cell reorientation in the presence of a field, cells are confined within microchannels to physically prohibit the alignment seen in 2D environment. Interestingly, we found that confinement results in an increase in cell velocity both in the absence and presence of an electric field compared to migration in 2D. Public Library of Science 2013-03-26 /pmc/articles/PMC3608730/ /pubmed/23555674 http://dx.doi.org/10.1371/journal.pone.0059447 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Yu-Ja Samorajski, Justin Kreimer, Rachel Searson, Peter C. The Influence of Electric Field and Confinement on Cell Motility |
title | The Influence of Electric Field and Confinement on Cell Motility |
title_full | The Influence of Electric Field and Confinement on Cell Motility |
title_fullStr | The Influence of Electric Field and Confinement on Cell Motility |
title_full_unstemmed | The Influence of Electric Field and Confinement on Cell Motility |
title_short | The Influence of Electric Field and Confinement on Cell Motility |
title_sort | influence of electric field and confinement on cell motility |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608730/ https://www.ncbi.nlm.nih.gov/pubmed/23555674 http://dx.doi.org/10.1371/journal.pone.0059447 |
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