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Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast

Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an m...

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Detalles Bibliográficos
Autores principales: Chan, Clara S., Jungreis, Irwin, Kellis, Manolis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609751/
https://www.ncbi.nlm.nih.gov/pubmed/23544069
http://dx.doi.org/10.1371/journal.pone.0059450
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author Chan, Clara S.
Jungreis, Irwin
Kellis, Manolis
author_facet Chan, Clara S.
Jungreis, Irwin
Kellis, Manolis
author_sort Chan, Clara S.
collection PubMed
description Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae.
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spelling pubmed-36097512013-03-29 Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast Chan, Clara S. Jungreis, Irwin Kellis, Manolis PLoS One Research Article Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae. Public Library of Science 2013-03-27 /pmc/articles/PMC3609751/ /pubmed/23544069 http://dx.doi.org/10.1371/journal.pone.0059450 Text en © 2013 Chan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chan, Clara S.
Jungreis, Irwin
Kellis, Manolis
Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title_full Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title_fullStr Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title_full_unstemmed Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title_short Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
title_sort heterologous stop codon readthrough of metazoan readthrough candidates in yeast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609751/
https://www.ncbi.nlm.nih.gov/pubmed/23544069
http://dx.doi.org/10.1371/journal.pone.0059450
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