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Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects

The trichothecene mycotoxin T-2 toxin is a common contaminant of food and feed and is also present in processed cereal derived products. Cytotoxic effects of T-2 toxin and its main metabolite HT-2 toxin are already well described with apoptosis being a major mechanism of action. However, effects on...

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Autores principales: Weidner, Maria, Hüwel, Sabine, Ebert, Franziska, Schwerdtle, Tanja, Galla, Hans-Joachim, Humpf, Hans-Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609806/
https://www.ncbi.nlm.nih.gov/pubmed/23544145
http://dx.doi.org/10.1371/journal.pone.0060484
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author Weidner, Maria
Hüwel, Sabine
Ebert, Franziska
Schwerdtle, Tanja
Galla, Hans-Joachim
Humpf, Hans-Ulrich
author_facet Weidner, Maria
Hüwel, Sabine
Ebert, Franziska
Schwerdtle, Tanja
Galla, Hans-Joachim
Humpf, Hans-Ulrich
author_sort Weidner, Maria
collection PubMed
description The trichothecene mycotoxin T-2 toxin is a common contaminant of food and feed and is also present in processed cereal derived products. Cytotoxic effects of T-2 toxin and its main metabolite HT-2 toxin are already well described with apoptosis being a major mechanism of action. However, effects on the central nervous system were until now only reported rarely. In this study we investigated the effects of T-2 and HT-2 toxin on the blood-brain barrier (BBB) in vitro. Besides strong cytotoxic effects on the BBB as determined by the CCK-8 assay, impairment of the barrier function starting at low nanomolar concentrations were observed for T-2 toxin. HT-2 toxin, however, caused barrier disruption at higher concentrations compared to T-2 toxin. Further, the influence on the tight junction protein occludin was studied and permeability of both toxins across the BBB was detected when applied from the apical (blood) or the basolateral (brain) side respectively. These results clearly indicate the ability of both toxins to enter the brain via the BBB.
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spelling pubmed-36098062013-03-29 Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects Weidner, Maria Hüwel, Sabine Ebert, Franziska Schwerdtle, Tanja Galla, Hans-Joachim Humpf, Hans-Ulrich PLoS One Research Article The trichothecene mycotoxin T-2 toxin is a common contaminant of food and feed and is also present in processed cereal derived products. Cytotoxic effects of T-2 toxin and its main metabolite HT-2 toxin are already well described with apoptosis being a major mechanism of action. However, effects on the central nervous system were until now only reported rarely. In this study we investigated the effects of T-2 and HT-2 toxin on the blood-brain barrier (BBB) in vitro. Besides strong cytotoxic effects on the BBB as determined by the CCK-8 assay, impairment of the barrier function starting at low nanomolar concentrations were observed for T-2 toxin. HT-2 toxin, however, caused barrier disruption at higher concentrations compared to T-2 toxin. Further, the influence on the tight junction protein occludin was studied and permeability of both toxins across the BBB was detected when applied from the apical (blood) or the basolateral (brain) side respectively. These results clearly indicate the ability of both toxins to enter the brain via the BBB. Public Library of Science 2013-03-27 /pmc/articles/PMC3609806/ /pubmed/23544145 http://dx.doi.org/10.1371/journal.pone.0060484 Text en © 2013 Weidner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Weidner, Maria
Hüwel, Sabine
Ebert, Franziska
Schwerdtle, Tanja
Galla, Hans-Joachim
Humpf, Hans-Ulrich
Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title_full Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title_fullStr Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title_full_unstemmed Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title_short Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects
title_sort influence of t-2 and ht-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609806/
https://www.ncbi.nlm.nih.gov/pubmed/23544145
http://dx.doi.org/10.1371/journal.pone.0060484
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