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Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes

PURPOSE: This study investigated which of the reciprocal stimuli between articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) played the more important role in enhancing cartilage matrix formation, and examined the relative importance of physical contact and soluble factors in the co-cultu...

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Autores principales: Zuo, Qiang, Cui, Weiding, Liu, Feng, Wang, Qing, Chen, Zhefeng, Fan, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609966/
https://www.ncbi.nlm.nih.gov/pubmed/23354690
http://dx.doi.org/10.1007/s00264-013-1782-z
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author Zuo, Qiang
Cui, Weiding
Liu, Feng
Wang, Qing
Chen, Zhefeng
Fan, Weimin
author_facet Zuo, Qiang
Cui, Weiding
Liu, Feng
Wang, Qing
Chen, Zhefeng
Fan, Weimin
author_sort Zuo, Qiang
collection PubMed
description PURPOSE: This study investigated which of the reciprocal stimuli between articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) played the more important role in enhancing cartilage matrix formation, and examined the relative importance of physical contact and soluble factors in the co-culture system. METHODS: Rat ACs and bone marrow MSCs with green fluorescent protein (GFP-BMSCs) were co-cultured in vitro with or without direct cell–cell contact at the ratio of 2:1. After co-culturing in direct cell–cell contact, ACs and GFP-BMSCs were separated by flow cytometry. The effects of different co-culture methods were analysed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. RESULTS: SOX-9, COL2 and aggrecan mRNA levels and protein expression in ACs co-cultured with direct cell–cell contact were significantly higher than in ACs co-cultured without direct cell–cell contact; and similar results were found in GFP-BMSCs. After co-culture either with or without direct cell–cell contact, mRNA levels and protein expression of SOX-9, COL2 and aggrecan in GFP-BMSCs were significantly lower than in ACs in the equivalent co-culture systems. Though the expression of chondrocyte-specific proteins in GFP-BMSCs was enhanced, the protein expression was still much lower than in ACs cultured alone. CONCLUSIONS: Reciprocal interactions exist between ACs and BMSCs in co-culture. The stimulating and supporting effects of BMSCs on ACs were more important in enhancing cartilage-matrix formation than the reciprocal effect of ACs on BMSCs. Both soluble factors and direct physical contact occur in AC/BMSC co-cultures, with physical contact playing a predominant, or at least very important role.
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spelling pubmed-36099662013-03-28 Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes Zuo, Qiang Cui, Weiding Liu, Feng Wang, Qing Chen, Zhefeng Fan, Weimin Int Orthop Original Paper PURPOSE: This study investigated which of the reciprocal stimuli between articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) played the more important role in enhancing cartilage matrix formation, and examined the relative importance of physical contact and soluble factors in the co-culture system. METHODS: Rat ACs and bone marrow MSCs with green fluorescent protein (GFP-BMSCs) were co-cultured in vitro with or without direct cell–cell contact at the ratio of 2:1. After co-culturing in direct cell–cell contact, ACs and GFP-BMSCs were separated by flow cytometry. The effects of different co-culture methods were analysed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. RESULTS: SOX-9, COL2 and aggrecan mRNA levels and protein expression in ACs co-cultured with direct cell–cell contact were significantly higher than in ACs co-cultured without direct cell–cell contact; and similar results were found in GFP-BMSCs. After co-culture either with or without direct cell–cell contact, mRNA levels and protein expression of SOX-9, COL2 and aggrecan in GFP-BMSCs were significantly lower than in ACs in the equivalent co-culture systems. Though the expression of chondrocyte-specific proteins in GFP-BMSCs was enhanced, the protein expression was still much lower than in ACs cultured alone. CONCLUSIONS: Reciprocal interactions exist between ACs and BMSCs in co-culture. The stimulating and supporting effects of BMSCs on ACs were more important in enhancing cartilage-matrix formation than the reciprocal effect of ACs on BMSCs. Both soluble factors and direct physical contact occur in AC/BMSC co-cultures, with physical contact playing a predominant, or at least very important role. Springer-Verlag 2013-01-25 2013-04 /pmc/articles/PMC3609966/ /pubmed/23354690 http://dx.doi.org/10.1007/s00264-013-1782-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Zuo, Qiang
Cui, Weiding
Liu, Feng
Wang, Qing
Chen, Zhefeng
Fan, Weimin
Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title_full Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title_fullStr Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title_full_unstemmed Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title_short Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
title_sort co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609966/
https://www.ncbi.nlm.nih.gov/pubmed/23354690
http://dx.doi.org/10.1007/s00264-013-1782-z
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