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TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine
Human T-cell Immunoglobulin and Mucin-domain containing proteins (TIM1, 3, and 4) specifically bind phosphatidylserine (PS). TIM1 has been proposed to serve as a cellular receptor for hepatitis A virus and Ebola virus and as an entry factor for dengue virus. Here we show that TIM1 promotes infection...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610696/ https://www.ncbi.nlm.nih.gov/pubmed/23555248 http://dx.doi.org/10.1371/journal.ppat.1003232 |
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author | Jemielity, Stephanie Wang, Jinyize J. Chan, Ying Kai Ahmed, Asim A. Li, Wenhui Monahan, Sheena Bu, Xia Farzan, Michael Freeman, Gordon J. Umetsu, Dale T. DeKruyff, Rosemarie H. Choe, Hyeryun |
author_facet | Jemielity, Stephanie Wang, Jinyize J. Chan, Ying Kai Ahmed, Asim A. Li, Wenhui Monahan, Sheena Bu, Xia Farzan, Michael Freeman, Gordon J. Umetsu, Dale T. DeKruyff, Rosemarie H. Choe, Hyeryun |
author_sort | Jemielity, Stephanie |
collection | PubMed |
description | Human T-cell Immunoglobulin and Mucin-domain containing proteins (TIM1, 3, and 4) specifically bind phosphatidylserine (PS). TIM1 has been proposed to serve as a cellular receptor for hepatitis A virus and Ebola virus and as an entry factor for dengue virus. Here we show that TIM1 promotes infection of retroviruses and virus-like particles (VLPs) pseudotyped with a range of viral entry proteins, in particular those from the filovirus, flavivirus, New World arenavirus and alphavirus families. TIM1 also robustly enhanced the infection of replication-competent viruses from the same families, including dengue, Tacaribe, Sindbis and Ross River viruses. All interactions between TIM1 and pseudoviruses or VLPs were PS-mediated, as demonstrated with liposome blocking and TIM1 mutagenesis experiments. In addition, other PS-binding proteins, such as Axl and TIM4, promoted infection similarly to TIM1. Finally, the blocking of PS receptors on macrophages inhibited the entry of Ebola VLPs, suggesting that PS receptors can contribute to infection in physiologically relevant cells. Notably, infection mediated by the entry proteins of Lassa fever virus, influenza A virus and SARS coronavirus was largely unaffected by TIM1 expression. Taken together our data show that TIM1 and related PS-binding proteins promote infection of diverse families of enveloped viruses, and may therefore be useful targets for broad-spectrum antiviral therapies. |
format | Online Article Text |
id | pubmed-3610696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36106962013-04-03 TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine Jemielity, Stephanie Wang, Jinyize J. Chan, Ying Kai Ahmed, Asim A. Li, Wenhui Monahan, Sheena Bu, Xia Farzan, Michael Freeman, Gordon J. Umetsu, Dale T. DeKruyff, Rosemarie H. Choe, Hyeryun PLoS Pathog Research Article Human T-cell Immunoglobulin and Mucin-domain containing proteins (TIM1, 3, and 4) specifically bind phosphatidylserine (PS). TIM1 has been proposed to serve as a cellular receptor for hepatitis A virus and Ebola virus and as an entry factor for dengue virus. Here we show that TIM1 promotes infection of retroviruses and virus-like particles (VLPs) pseudotyped with a range of viral entry proteins, in particular those from the filovirus, flavivirus, New World arenavirus and alphavirus families. TIM1 also robustly enhanced the infection of replication-competent viruses from the same families, including dengue, Tacaribe, Sindbis and Ross River viruses. All interactions between TIM1 and pseudoviruses or VLPs were PS-mediated, as demonstrated with liposome blocking and TIM1 mutagenesis experiments. In addition, other PS-binding proteins, such as Axl and TIM4, promoted infection similarly to TIM1. Finally, the blocking of PS receptors on macrophages inhibited the entry of Ebola VLPs, suggesting that PS receptors can contribute to infection in physiologically relevant cells. Notably, infection mediated by the entry proteins of Lassa fever virus, influenza A virus and SARS coronavirus was largely unaffected by TIM1 expression. Taken together our data show that TIM1 and related PS-binding proteins promote infection of diverse families of enveloped viruses, and may therefore be useful targets for broad-spectrum antiviral therapies. Public Library of Science 2013-03-28 /pmc/articles/PMC3610696/ /pubmed/23555248 http://dx.doi.org/10.1371/journal.ppat.1003232 Text en © 2013 Jemielity et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jemielity, Stephanie Wang, Jinyize J. Chan, Ying Kai Ahmed, Asim A. Li, Wenhui Monahan, Sheena Bu, Xia Farzan, Michael Freeman, Gordon J. Umetsu, Dale T. DeKruyff, Rosemarie H. Choe, Hyeryun TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title | TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title_full | TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title_fullStr | TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title_full_unstemmed | TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title_short | TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine |
title_sort | tim-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610696/ https://www.ncbi.nlm.nih.gov/pubmed/23555248 http://dx.doi.org/10.1371/journal.ppat.1003232 |
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