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Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung

Pneumococcal carriage is both immunising and a pre-requisite for mucosal and systemic disease. Murine models of pneumococcal colonisation show that IL-17A-secreting CD4(+) T-cells (Th-17 cells) are essential for clearance of pneumococci from the nasopharynx. Pneumococcal-responding IL-17A-secreting...

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Autores principales: Wright, Adam K. A., Bangert, Mathieu, Gritzfeld, Jenna F., Ferreira, Daniela M., Jambo, Kondwani C., Wright, Angela D., Collins, Andrea M., Gordon, Stephen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610738/
https://www.ncbi.nlm.nih.gov/pubmed/23555269
http://dx.doi.org/10.1371/journal.ppat.1003274
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author Wright, Adam K. A.
Bangert, Mathieu
Gritzfeld, Jenna F.
Ferreira, Daniela M.
Jambo, Kondwani C.
Wright, Angela D.
Collins, Andrea M.
Gordon, Stephen B.
author_facet Wright, Adam K. A.
Bangert, Mathieu
Gritzfeld, Jenna F.
Ferreira, Daniela M.
Jambo, Kondwani C.
Wright, Angela D.
Collins, Andrea M.
Gordon, Stephen B.
author_sort Wright, Adam K. A.
collection PubMed
description Pneumococcal carriage is both immunising and a pre-requisite for mucosal and systemic disease. Murine models of pneumococcal colonisation show that IL-17A-secreting CD4(+) T-cells (Th-17 cells) are essential for clearance of pneumococci from the nasopharynx. Pneumococcal-responding IL-17A-secreting CD4(+) T-cells have not been described in the adult human lung and it is unknown whether they can be elicited by carriage and protect the lung from pneumococcal infection. We investigated the direct effect of experimental human pneumococcal nasal carriage (EHPC) on the frequency and phenotype of cognate CD4(+) T-cells in broncho-alveolar lavage and blood using multi-parameter flow cytometry. We then examined whether they could augment ex vivo alveolar macrophage killing of pneumococci using an in vitro assay. We showed that human pneumococcal carriage leads to a 17.4-fold (p = 0.007) and 8-fold (p = 0.003) increase in the frequency of cognate IL-17A(+) CD4(+) T-cells in BAL and blood, respectively. The phenotype with the largest proportion were TNF(+)/IL-17A(+) co-producing CD4(+) memory T-cells (p<0.01); IFNγ(+) CD4(+) memory T-cells were not significantly increased following carriage. Pneumococci could stimulate large amounts of IL-17A protein from BAL cells in the absence of carriage but in the presence of cognate CD4(+) memory T-cells, IL-17A protein levels were increased by a further 50%. Further to this we then show that alveolar macrophages, which express IL-17A receptors A and C, showed enhanced killing of opsonised pneumococci when stimulated with rhIL-17A (p = 0.013). Killing negatively correlated with RC (r = −0.9, p = 0.017) but not RA expression. We conclude that human pneumococcal carriage can increase the proportion of lung IL-17A-secreting CD4(+) memory T-cells that may enhance innate cellular immunity against pathogenic challenge. These pathways may be utilised to enhance vaccine efficacy to protect the lung against pneumonia.
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spelling pubmed-36107382013-04-03 Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung Wright, Adam K. A. Bangert, Mathieu Gritzfeld, Jenna F. Ferreira, Daniela M. Jambo, Kondwani C. Wright, Angela D. Collins, Andrea M. Gordon, Stephen B. PLoS Pathog Research Article Pneumococcal carriage is both immunising and a pre-requisite for mucosal and systemic disease. Murine models of pneumococcal colonisation show that IL-17A-secreting CD4(+) T-cells (Th-17 cells) are essential for clearance of pneumococci from the nasopharynx. Pneumococcal-responding IL-17A-secreting CD4(+) T-cells have not been described in the adult human lung and it is unknown whether they can be elicited by carriage and protect the lung from pneumococcal infection. We investigated the direct effect of experimental human pneumococcal nasal carriage (EHPC) on the frequency and phenotype of cognate CD4(+) T-cells in broncho-alveolar lavage and blood using multi-parameter flow cytometry. We then examined whether they could augment ex vivo alveolar macrophage killing of pneumococci using an in vitro assay. We showed that human pneumococcal carriage leads to a 17.4-fold (p = 0.007) and 8-fold (p = 0.003) increase in the frequency of cognate IL-17A(+) CD4(+) T-cells in BAL and blood, respectively. The phenotype with the largest proportion were TNF(+)/IL-17A(+) co-producing CD4(+) memory T-cells (p<0.01); IFNγ(+) CD4(+) memory T-cells were not significantly increased following carriage. Pneumococci could stimulate large amounts of IL-17A protein from BAL cells in the absence of carriage but in the presence of cognate CD4(+) memory T-cells, IL-17A protein levels were increased by a further 50%. Further to this we then show that alveolar macrophages, which express IL-17A receptors A and C, showed enhanced killing of opsonised pneumococci when stimulated with rhIL-17A (p = 0.013). Killing negatively correlated with RC (r = −0.9, p = 0.017) but not RA expression. We conclude that human pneumococcal carriage can increase the proportion of lung IL-17A-secreting CD4(+) memory T-cells that may enhance innate cellular immunity against pathogenic challenge. These pathways may be utilised to enhance vaccine efficacy to protect the lung against pneumonia. Public Library of Science 2013-03-28 /pmc/articles/PMC3610738/ /pubmed/23555269 http://dx.doi.org/10.1371/journal.ppat.1003274 Text en © 2013 Wright et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wright, Adam K. A.
Bangert, Mathieu
Gritzfeld, Jenna F.
Ferreira, Daniela M.
Jambo, Kondwani C.
Wright, Angela D.
Collins, Andrea M.
Gordon, Stephen B.
Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title_full Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title_fullStr Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title_full_unstemmed Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title_short Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung
title_sort experimental human pneumococcal carriage augments il-17a-dependent t-cell defence of the lung
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610738/
https://www.ncbi.nlm.nih.gov/pubmed/23555269
http://dx.doi.org/10.1371/journal.ppat.1003274
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