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Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)

We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective phage-display approach in which only a limited set of larger fragmen...

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Autores principales: Gabrielli, Federico, Salvi, Roberto, Garulli, Chiara, Kalogris, Cristina, Arima, Serena, Tardella, Luca, Monaci, Paolo, Pupa, Serenella M., Tagliabue, Elda, Montani, Maura, Quaglino, Elena, Stramucci, Lorenzo, Curcio, Claudia, Marchini, Cristina, Amici, Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610777/
https://www.ncbi.nlm.nih.gov/pubmed/23555577
http://dx.doi.org/10.1371/journal.pone.0058358
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author Gabrielli, Federico
Salvi, Roberto
Garulli, Chiara
Kalogris, Cristina
Arima, Serena
Tardella, Luca
Monaci, Paolo
Pupa, Serenella M.
Tagliabue, Elda
Montani, Maura
Quaglino, Elena
Stramucci, Lorenzo
Curcio, Claudia
Marchini, Cristina
Amici, Augusto
author_facet Gabrielli, Federico
Salvi, Roberto
Garulli, Chiara
Kalogris, Cristina
Arima, Serena
Tardella, Luca
Monaci, Paolo
Pupa, Serenella M.
Tagliabue, Elda
Montani, Maura
Quaglino, Elena
Stramucci, Lorenzo
Curcio, Claudia
Marchini, Cristina
Amici, Augusto
author_sort Gabrielli, Federico
collection PubMed
description We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective phage-display approach in which only a limited set of larger fragments (about 100 aa in length) are expressed on the phage surface. Using the human HER2 oncoprotein as a target, we identified novel B-cell conformational epitopes. The same homologous epitopes were also detected in rat HER2 and all corresponded to the epitopes predicted by computational analysis (PEPITO software), showing that LFPD gives reproducible and accurate results. Interestingly, these newly identified HER2 epitopes seem to be crucial for an effective immune response against HER2-overexpressing breast cancers and might help discriminating between metastatic breast cancer and early breast cancer patients. Overall, the results obtained in this study demonstrated the utility of LFPD and its potential application to the detection of conformational epitopes on many other molecules of interest, as well as, the development of new and potentially more effective B-cell conformational epitopes based vaccines.
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spelling pubmed-36107772013-04-03 Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD) Gabrielli, Federico Salvi, Roberto Garulli, Chiara Kalogris, Cristina Arima, Serena Tardella, Luca Monaci, Paolo Pupa, Serenella M. Tagliabue, Elda Montani, Maura Quaglino, Elena Stramucci, Lorenzo Curcio, Claudia Marchini, Cristina Amici, Augusto PLoS One Research Article We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective phage-display approach in which only a limited set of larger fragments (about 100 aa in length) are expressed on the phage surface. Using the human HER2 oncoprotein as a target, we identified novel B-cell conformational epitopes. The same homologous epitopes were also detected in rat HER2 and all corresponded to the epitopes predicted by computational analysis (PEPITO software), showing that LFPD gives reproducible and accurate results. Interestingly, these newly identified HER2 epitopes seem to be crucial for an effective immune response against HER2-overexpressing breast cancers and might help discriminating between metastatic breast cancer and early breast cancer patients. Overall, the results obtained in this study demonstrated the utility of LFPD and its potential application to the detection of conformational epitopes on many other molecules of interest, as well as, the development of new and potentially more effective B-cell conformational epitopes based vaccines. Public Library of Science 2013-03-28 /pmc/articles/PMC3610777/ /pubmed/23555577 http://dx.doi.org/10.1371/journal.pone.0058358 Text en © 2013 Gabrielli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gabrielli, Federico
Salvi, Roberto
Garulli, Chiara
Kalogris, Cristina
Arima, Serena
Tardella, Luca
Monaci, Paolo
Pupa, Serenella M.
Tagliabue, Elda
Montani, Maura
Quaglino, Elena
Stramucci, Lorenzo
Curcio, Claudia
Marchini, Cristina
Amici, Augusto
Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title_full Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title_fullStr Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title_full_unstemmed Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title_short Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
title_sort identification of relevant conformational epitopes on the her2 oncoprotein by using large fragment phage display (lfpd)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610777/
https://www.ncbi.nlm.nih.gov/pubmed/23555577
http://dx.doi.org/10.1371/journal.pone.0058358
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