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Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway
Oxidative damage plays a critical role in many diseases of the central nervous system. This study was conducted to determine the molecular mechanisms involved in the putative anti-oxidative effects of curcumin against experimental stroke. Oxygen and glucose deprivation/reoxygenation (OGD/R) was used...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610879/ https://www.ncbi.nlm.nih.gov/pubmed/23555802 http://dx.doi.org/10.1371/journal.pone.0059843 |
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author | Wu, Jingxian Li, Qiong Wang, Xiaoyan Yu, Shanshan Li, Lan Wu, Xuemei Chen, Yanlin Zhao, Jing Zhao, Yong |
author_facet | Wu, Jingxian Li, Qiong Wang, Xiaoyan Yu, Shanshan Li, Lan Wu, Xuemei Chen, Yanlin Zhao, Jing Zhao, Yong |
author_sort | Wu, Jingxian |
collection | PubMed |
description | Oxidative damage plays a critical role in many diseases of the central nervous system. This study was conducted to determine the molecular mechanisms involved in the putative anti-oxidative effects of curcumin against experimental stroke. Oxygen and glucose deprivation/reoxygenation (OGD/R) was used to mimic ischemic insult in primary cultured cortical neurons. A rapid increase in the intracellular expression of NAD(P)H: quinone oxidoreductase1 (NQO1) induced by OGD was counteracted by curcumin post-treatment, which paralleled attenuated cell injury. The reduction of phosphorylation Akt induced by OGD was restored by curcumin. Consequently, NQO1 expression and the binding activity of nuclear factor-erythroid 2-related factor 2 (Nrf2) to antioxidant response element (ARE) were increased. LY294002 blocked the increase in phospho-Akt evoked by curcumin and abolished the associated protective effect. Adult male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion for 60 minutes. Curcumin administration significantly reduced infarct size. Curcumin also markedly reduced oxidative stress levels in middle cerebral artery occlusion (MCAO) rats; hence, these effects were all suppressed by LY294002. Taken together, these findings provide evidence that curcumin protects neurons against ischemic injury, and this neuroprotective effect involves the Akt/Nrf2 pathway. In addition, Nrf2 is involved in the neuroprotective effects of curcumin against oxidative damage. |
format | Online Article Text |
id | pubmed-3610879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36108792013-04-03 Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway Wu, Jingxian Li, Qiong Wang, Xiaoyan Yu, Shanshan Li, Lan Wu, Xuemei Chen, Yanlin Zhao, Jing Zhao, Yong PLoS One Research Article Oxidative damage plays a critical role in many diseases of the central nervous system. This study was conducted to determine the molecular mechanisms involved in the putative anti-oxidative effects of curcumin against experimental stroke. Oxygen and glucose deprivation/reoxygenation (OGD/R) was used to mimic ischemic insult in primary cultured cortical neurons. A rapid increase in the intracellular expression of NAD(P)H: quinone oxidoreductase1 (NQO1) induced by OGD was counteracted by curcumin post-treatment, which paralleled attenuated cell injury. The reduction of phosphorylation Akt induced by OGD was restored by curcumin. Consequently, NQO1 expression and the binding activity of nuclear factor-erythroid 2-related factor 2 (Nrf2) to antioxidant response element (ARE) were increased. LY294002 blocked the increase in phospho-Akt evoked by curcumin and abolished the associated protective effect. Adult male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion for 60 minutes. Curcumin administration significantly reduced infarct size. Curcumin also markedly reduced oxidative stress levels in middle cerebral artery occlusion (MCAO) rats; hence, these effects were all suppressed by LY294002. Taken together, these findings provide evidence that curcumin protects neurons against ischemic injury, and this neuroprotective effect involves the Akt/Nrf2 pathway. In addition, Nrf2 is involved in the neuroprotective effects of curcumin against oxidative damage. Public Library of Science 2013-03-28 /pmc/articles/PMC3610879/ /pubmed/23555802 http://dx.doi.org/10.1371/journal.pone.0059843 Text en © 2013 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Jingxian Li, Qiong Wang, Xiaoyan Yu, Shanshan Li, Lan Wu, Xuemei Chen, Yanlin Zhao, Jing Zhao, Yong Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title | Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title_full | Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title_fullStr | Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title_full_unstemmed | Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title_short | Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway |
title_sort | neuroprotection by curcumin in ischemic brain injury involves the akt/nrf2 pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610879/ https://www.ncbi.nlm.nih.gov/pubmed/23555802 http://dx.doi.org/10.1371/journal.pone.0059843 |
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