Specific In Vivo Labeling of Tyrosinated α-Tubulin and Measurement of Microtubule Dynamics Using a GFP Tagged, Cytoplasmically Expressed Recombinant Antibody

GFP-tagged proteins are used extensively as biosensors for protein localization and function, but the GFP moiety can interfere with protein properties. An alternative is to indirectly label proteins using intracellular recombinant antibodies (scFvs), but most antibody fragments are insoluble in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Cassimeris, Lynne, Guglielmi, Laurence, Denis, Vincent, Larroque, Christian, Martineau, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610906/
https://www.ncbi.nlm.nih.gov/pubmed/23555790
http://dx.doi.org/10.1371/journal.pone.0059812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610906/
https://www.ncbi.nlm.nih.gov/pubmed/23555790
http://dx.doi.org/10.1371/journal.pone.0059812

Ejemplares similares