Detection of Streptococcus equi subspecies equi using a triplex qPCR assay

Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 p...

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Detalles Bibliográficos
Autores principales: Webb, Katy, Barker, Colin, Harrison, Tihana, Heather, Zoe, Steward, Karen F., Robinson, Carl, Newton, J. Richard, Waller, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Balliere Tindall 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3611602/
https://www.ncbi.nlm.nih.gov/pubmed/22884566
http://dx.doi.org/10.1016/j.tvjl.2012.07.007
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author Webb, Katy
Barker, Colin
Harrison, Tihana
Heather, Zoe
Steward, Karen F.
Robinson, Carl
Newton, J. Richard
Waller, Andrew S.
author_facet Webb, Katy
Barker, Colin
Harrison, Tihana
Heather, Zoe
Steward, Karen F.
Robinson, Carl
Newton, J. Richard
Waller, Andrew S.
author_sort Webb, Katy
collection PubMed
description Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
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spelling pubmed-36116022013-03-29 Detection of Streptococcus equi subspecies equi using a triplex qPCR assay Webb, Katy Barker, Colin Harrison, Tihana Heather, Zoe Steward, Karen F. Robinson, Carl Newton, J. Richard Waller, Andrew S. Vet J Article Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria. Balliere Tindall 2013-03 /pmc/articles/PMC3611602/ /pubmed/22884566 http://dx.doi.org/10.1016/j.tvjl.2012.07.007 Text en © 2013 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Webb, Katy
Barker, Colin
Harrison, Tihana
Heather, Zoe
Steward, Karen F.
Robinson, Carl
Newton, J. Richard
Waller, Andrew S.
Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title_full Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title_fullStr Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title_full_unstemmed Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title_short Detection of Streptococcus equi subspecies equi using a triplex qPCR assay
title_sort detection of streptococcus equi subspecies equi using a triplex qpcr assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3611602/
https://www.ncbi.nlm.nih.gov/pubmed/22884566
http://dx.doi.org/10.1016/j.tvjl.2012.07.007
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