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Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.

Cissus repanda Vahl. belongs to the family Vitaceae, commonly known in Hindi as “Panivel,” is a large climber distributed all over India. The crushed or powder of root is prescribed by tribal people and traditional medical practitioners of Orissa for its healing properties in cases of bone fracture,...

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Autores principales: Harisha, C. R., Acharya, Rabinarayan, Chauhan, Maltiben G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3611653/
https://www.ncbi.nlm.nih.gov/pubmed/23559804
http://dx.doi.org/10.4103/0974-8520.105252
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author Harisha, C. R.
Acharya, Rabinarayan
Chauhan, Maltiben G.
author_facet Harisha, C. R.
Acharya, Rabinarayan
Chauhan, Maltiben G.
author_sort Harisha, C. R.
collection PubMed
description Cissus repanda Vahl. belongs to the family Vitaceae, commonly known in Hindi as “Panivel,” is a large climber distributed all over India. The crushed or powder of root is prescribed by tribal people and traditional medical practitioners of Orissa for its healing properties in cases of bone fracture, cuts and wounds, swellings, and so on. In spite of its reputation, its leaves have not been investigated scientifically. The present study deals with pharmacognostical and molecular characterization by Random Amplified Polymorphic DNA (RAPD) markers and their role in laying down standardization and pharmacopoeial parameters. Genomic isolation of DNA from fresh leaves was amplified by RAPD markers. The diagnostic characters are mucilage, calcium oxalate rosette crystals, spiral vessels, and fibers. The unique bands obtained in Polymerase Chain Reaction (PCR) amplification clearly discriminated having, many bright and light bands indicating the genuinity of the plant. RAPD may serve as a complementary tool in quality control of many herbal sources.
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spelling pubmed-36116532013-04-04 Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl. Harisha, C. R. Acharya, Rabinarayan Chauhan, Maltiben G. Ayu Pharmaceutical Standardization Cissus repanda Vahl. belongs to the family Vitaceae, commonly known in Hindi as “Panivel,” is a large climber distributed all over India. The crushed or powder of root is prescribed by tribal people and traditional medical practitioners of Orissa for its healing properties in cases of bone fracture, cuts and wounds, swellings, and so on. In spite of its reputation, its leaves have not been investigated scientifically. The present study deals with pharmacognostical and molecular characterization by Random Amplified Polymorphic DNA (RAPD) markers and their role in laying down standardization and pharmacopoeial parameters. Genomic isolation of DNA from fresh leaves was amplified by RAPD markers. The diagnostic characters are mucilage, calcium oxalate rosette crystals, spiral vessels, and fibers. The unique bands obtained in Polymerase Chain Reaction (PCR) amplification clearly discriminated having, many bright and light bands indicating the genuinity of the plant. RAPD may serve as a complementary tool in quality control of many herbal sources. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3611653/ /pubmed/23559804 http://dx.doi.org/10.4103/0974-8520.105252 Text en Copyright: © AYU (An International Quarterly Journal of Research in Ayurveda) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Pharmaceutical Standardization
Harisha, C. R.
Acharya, Rabinarayan
Chauhan, Maltiben G.
Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title_full Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title_fullStr Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title_full_unstemmed Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title_short Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl.
title_sort development of random amplified polymorphic dna markers for authentification of cissus repanda vahl.
topic Pharmaceutical Standardization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3611653/
https://www.ncbi.nlm.nih.gov/pubmed/23559804
http://dx.doi.org/10.4103/0974-8520.105252
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