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Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection

Primary human cytomegalovirus (HCMV) infections during pregnancy are associated with a high risk of virus transmission to the fetus. To identify correlates of intrauterine HCMV transmission, serial serum samples from HCMV transmitter and non-transmitter pregnant women with primary HCMV infection wer...

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Autores principales: Lilleri, Daniele, Kabanova, Anna, Revello, Maria Grazia, Percivalle, Elena, Sarasini, Antonella, Genini, Emilia, Sallusto, Federica, Lanzavecchia, Antonio, Corti, Davide, Gerna, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612069/
https://www.ncbi.nlm.nih.gov/pubmed/23555812
http://dx.doi.org/10.1371/journal.pone.0059863
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author Lilleri, Daniele
Kabanova, Anna
Revello, Maria Grazia
Percivalle, Elena
Sarasini, Antonella
Genini, Emilia
Sallusto, Federica
Lanzavecchia, Antonio
Corti, Davide
Gerna, Giuseppe
author_facet Lilleri, Daniele
Kabanova, Anna
Revello, Maria Grazia
Percivalle, Elena
Sarasini, Antonella
Genini, Emilia
Sallusto, Federica
Lanzavecchia, Antonio
Corti, Davide
Gerna, Giuseppe
author_sort Lilleri, Daniele
collection PubMed
description Primary human cytomegalovirus (HCMV) infections during pregnancy are associated with a high risk of virus transmission to the fetus. To identify correlates of intrauterine HCMV transmission, serial serum samples from HCMV transmitter and non-transmitter pregnant women with primary HCMV infection were analyzed for the presence of neutralizing antibodies against different glycoproteins and glycoprotein complexes, which are known to mediate entry into distinct types of host cells. Neutralizing activity was detected in the sera early after primary infection; absorption with a soluble pentameric complex formed by gH/gL/pUL128-131, but not with gH/gL dimer or with gB, abolished the capacity of sera to neutralize infection of epithelial cells. Importantly, an early, high antibody response to pentamer antigenic sites was associated with a significantly reduced risk of HCMV transmission to the fetus. This association is consistent with the high in vitro inhibition of HCMV infection of epithelial/endothelial cells as well as cell-to-cell spreading and virus transfer to leukocytes by anti-pentamer antibodies. Taken together, these findings indicate that the HCMV pentamer complex is a major target of the antibody-mediated maternal immunity.
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spelling pubmed-36120692013-04-03 Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection Lilleri, Daniele Kabanova, Anna Revello, Maria Grazia Percivalle, Elena Sarasini, Antonella Genini, Emilia Sallusto, Federica Lanzavecchia, Antonio Corti, Davide Gerna, Giuseppe PLoS One Research Article Primary human cytomegalovirus (HCMV) infections during pregnancy are associated with a high risk of virus transmission to the fetus. To identify correlates of intrauterine HCMV transmission, serial serum samples from HCMV transmitter and non-transmitter pregnant women with primary HCMV infection were analyzed for the presence of neutralizing antibodies against different glycoproteins and glycoprotein complexes, which are known to mediate entry into distinct types of host cells. Neutralizing activity was detected in the sera early after primary infection; absorption with a soluble pentameric complex formed by gH/gL/pUL128-131, but not with gH/gL dimer or with gB, abolished the capacity of sera to neutralize infection of epithelial cells. Importantly, an early, high antibody response to pentamer antigenic sites was associated with a significantly reduced risk of HCMV transmission to the fetus. This association is consistent with the high in vitro inhibition of HCMV infection of epithelial/endothelial cells as well as cell-to-cell spreading and virus transfer to leukocytes by anti-pentamer antibodies. Taken together, these findings indicate that the HCMV pentamer complex is a major target of the antibody-mediated maternal immunity. Public Library of Science 2013-03-29 /pmc/articles/PMC3612069/ /pubmed/23555812 http://dx.doi.org/10.1371/journal.pone.0059863 Text en © 2013 Lilleri et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lilleri, Daniele
Kabanova, Anna
Revello, Maria Grazia
Percivalle, Elena
Sarasini, Antonella
Genini, Emilia
Sallusto, Federica
Lanzavecchia, Antonio
Corti, Davide
Gerna, Giuseppe
Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title_full Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title_fullStr Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title_full_unstemmed Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title_short Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection
title_sort fetal human cytomegalovirus transmission correlates with delayed maternal antibodies to gh/gl/pul128-130-131 complex during primary infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612069/
https://www.ncbi.nlm.nih.gov/pubmed/23555812
http://dx.doi.org/10.1371/journal.pone.0059863
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