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The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma

Our previous study has found that the abundance of peritumoral CD68(+) macrophages was associated with poor prognosis in hepatocellular carcinoma (HCC) after resection. However, CD68 staining could not discriminate the protumoral or tumoricidal subpopulations from pan-macrophages. CD163 is a marker...

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Autores principales: Kong, Ling-Qun, Zhu, Xiao-Dong, Xu, Hua-Xiang, Zhang, Ju-Bo, Lu, Lu, Wang, Wen-Quan, Zhang, Qiang-Bo, Wu, Wei-Zhong, Wang, Lu, Fan, Jia, Tang, Zhao-You, Sun, Hui-Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612077/
https://www.ncbi.nlm.nih.gov/pubmed/23555776
http://dx.doi.org/10.1371/journal.pone.0059771
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author Kong, Ling-Qun
Zhu, Xiao-Dong
Xu, Hua-Xiang
Zhang, Ju-Bo
Lu, Lu
Wang, Wen-Quan
Zhang, Qiang-Bo
Wu, Wei-Zhong
Wang, Lu
Fan, Jia
Tang, Zhao-You
Sun, Hui-Chuan
author_facet Kong, Ling-Qun
Zhu, Xiao-Dong
Xu, Hua-Xiang
Zhang, Ju-Bo
Lu, Lu
Wang, Wen-Quan
Zhang, Qiang-Bo
Wu, Wei-Zhong
Wang, Lu
Fan, Jia
Tang, Zhao-You
Sun, Hui-Chuan
author_sort Kong, Ling-Qun
collection PubMed
description Our previous study has found that the abundance of peritumoral CD68(+) macrophages was associated with poor prognosis in hepatocellular carcinoma (HCC) after resection. However, CD68 staining could not discriminate the protumoral or tumoricidal subpopulations from pan-macrophages. CD163 is a marker of alternatively activated macrophages. In this study, the clinical significance of CD163(+) cells in tumors and peritumoral liver tissues was evaluated in a cohort of 295 patients with HCC after curative resection. We found that the density of CD163(+) cells was well correlated with that of CD68(+) cells in both tumors and peritumoral liver tissues but was much more. Immunostaining on consecutive sections and flow cytometry assay on surgical resected specimens further supported the findings that the CD163(+) cells was more abundant than CD68(+) cells. The density of peritumoral CD68(+) cells was associated with poor recurrence-free survival (RFS) and poor overall survival (OS) (P = 0.004 and P = 0.001, respectively), whereas the CD163(+) cells have no prognostic values either in tumors or in peritumoral liver tissues. In another cohort of 107 HCC patients, preoperative plasma concentration of soluble form of CD163 (sCD163) was associated with active hepatitis-related factors but not associated with the markers of tumor invasion. In conclusion, both the CD163(+) cells local infiltration and plasma sCD163 were of limited significance in HCC, and they were more likely markers related to active hepatitis rather than tumor progression.
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spelling pubmed-36120772013-04-03 The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma Kong, Ling-Qun Zhu, Xiao-Dong Xu, Hua-Xiang Zhang, Ju-Bo Lu, Lu Wang, Wen-Quan Zhang, Qiang-Bo Wu, Wei-Zhong Wang, Lu Fan, Jia Tang, Zhao-You Sun, Hui-Chuan PLoS One Research Article Our previous study has found that the abundance of peritumoral CD68(+) macrophages was associated with poor prognosis in hepatocellular carcinoma (HCC) after resection. However, CD68 staining could not discriminate the protumoral or tumoricidal subpopulations from pan-macrophages. CD163 is a marker of alternatively activated macrophages. In this study, the clinical significance of CD163(+) cells in tumors and peritumoral liver tissues was evaluated in a cohort of 295 patients with HCC after curative resection. We found that the density of CD163(+) cells was well correlated with that of CD68(+) cells in both tumors and peritumoral liver tissues but was much more. Immunostaining on consecutive sections and flow cytometry assay on surgical resected specimens further supported the findings that the CD163(+) cells was more abundant than CD68(+) cells. The density of peritumoral CD68(+) cells was associated with poor recurrence-free survival (RFS) and poor overall survival (OS) (P = 0.004 and P = 0.001, respectively), whereas the CD163(+) cells have no prognostic values either in tumors or in peritumoral liver tissues. In another cohort of 107 HCC patients, preoperative plasma concentration of soluble form of CD163 (sCD163) was associated with active hepatitis-related factors but not associated with the markers of tumor invasion. In conclusion, both the CD163(+) cells local infiltration and plasma sCD163 were of limited significance in HCC, and they were more likely markers related to active hepatitis rather than tumor progression. Public Library of Science 2013-03-29 /pmc/articles/PMC3612077/ /pubmed/23555776 http://dx.doi.org/10.1371/journal.pone.0059771 Text en © 2013 Kong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kong, Ling-Qun
Zhu, Xiao-Dong
Xu, Hua-Xiang
Zhang, Ju-Bo
Lu, Lu
Wang, Wen-Quan
Zhang, Qiang-Bo
Wu, Wei-Zhong
Wang, Lu
Fan, Jia
Tang, Zhao-You
Sun, Hui-Chuan
The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title_full The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title_fullStr The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title_full_unstemmed The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title_short The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma
title_sort clinical significance of the cd163+ and cd68+ macrophages in patients with hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612077/
https://www.ncbi.nlm.nih.gov/pubmed/23555776
http://dx.doi.org/10.1371/journal.pone.0059771
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