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Hemogenic endocardium contributes to transient definitive hematopoiesis

Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian...

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Autores principales: Nakano, Haruko, Liu, Xiaoqian, Arshi, Armin, Nakashima, Yasuhiro, van Handel, Ben, Sasidharan, Rajkumar, Harmon, Andrew W., Shin, Jae-Ho, Schwartz, Robert J., Conway, Simon J., Harvey, Richard P., Pashmforoush, Mohammad, Mikkola, Hanna K. A., Nakano, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612528/
https://www.ncbi.nlm.nih.gov/pubmed/23463007
http://dx.doi.org/10.1038/ncomms2569
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author Nakano, Haruko
Nakano, Haruko
Liu, Xiaoqian
Arshi, Armin
Nakashima, Yasuhiro
van Handel, Ben
Sasidharan, Rajkumar
Harmon, Andrew W.
Shin, Jae-Ho
Schwartz, Robert J.
Conway, Simon J.
Harvey, Richard P.
Pashmforoush, Mohammad
Mikkola, Hanna K. A.
Nakano, Atsushi
author_facet Nakano, Haruko
Nakano, Haruko
Liu, Xiaoqian
Arshi, Armin
Nakashima, Yasuhiro
van Handel, Ben
Sasidharan, Rajkumar
Harmon, Andrew W.
Shin, Jae-Ho
Schwartz, Robert J.
Conway, Simon J.
Harvey, Richard P.
Pashmforoush, Mohammad
Mikkola, Hanna K. A.
Nakano, Atsushi
author_sort Nakano, Haruko
collection PubMed
description Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a hemogenic organ akin to the dorsal aorta. Here we examine the hemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Hemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the hemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells expressing cardiac markers serve as a de novo source for transient definitive hematopoietic progenitors.
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spelling pubmed-36125282013-09-05 Hemogenic endocardium contributes to transient definitive hematopoiesis Nakano, Haruko Nakano, Haruko Liu, Xiaoqian Arshi, Armin Nakashima, Yasuhiro van Handel, Ben Sasidharan, Rajkumar Harmon, Andrew W. Shin, Jae-Ho Schwartz, Robert J. Conway, Simon J. Harvey, Richard P. Pashmforoush, Mohammad Mikkola, Hanna K. A. Nakano, Atsushi Nat Commun Article Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a hemogenic organ akin to the dorsal aorta. Here we examine the hemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Hemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the hemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells expressing cardiac markers serve as a de novo source for transient definitive hematopoietic progenitors. 2013 /pmc/articles/PMC3612528/ /pubmed/23463007 http://dx.doi.org/10.1038/ncomms2569 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Nakano, Haruko
Nakano, Haruko
Liu, Xiaoqian
Arshi, Armin
Nakashima, Yasuhiro
van Handel, Ben
Sasidharan, Rajkumar
Harmon, Andrew W.
Shin, Jae-Ho
Schwartz, Robert J.
Conway, Simon J.
Harvey, Richard P.
Pashmforoush, Mohammad
Mikkola, Hanna K. A.
Nakano, Atsushi
Hemogenic endocardium contributes to transient definitive hematopoiesis
title Hemogenic endocardium contributes to transient definitive hematopoiesis
title_full Hemogenic endocardium contributes to transient definitive hematopoiesis
title_fullStr Hemogenic endocardium contributes to transient definitive hematopoiesis
title_full_unstemmed Hemogenic endocardium contributes to transient definitive hematopoiesis
title_short Hemogenic endocardium contributes to transient definitive hematopoiesis
title_sort hemogenic endocardium contributes to transient definitive hematopoiesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612528/
https://www.ncbi.nlm.nih.gov/pubmed/23463007
http://dx.doi.org/10.1038/ncomms2569
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