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Hemogenic endocardium contributes to transient definitive hematopoiesis
Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612528/ https://www.ncbi.nlm.nih.gov/pubmed/23463007 http://dx.doi.org/10.1038/ncomms2569 |
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author | Nakano, Haruko Nakano, Haruko Liu, Xiaoqian Arshi, Armin Nakashima, Yasuhiro van Handel, Ben Sasidharan, Rajkumar Harmon, Andrew W. Shin, Jae-Ho Schwartz, Robert J. Conway, Simon J. Harvey, Richard P. Pashmforoush, Mohammad Mikkola, Hanna K. A. Nakano, Atsushi |
author_facet | Nakano, Haruko Nakano, Haruko Liu, Xiaoqian Arshi, Armin Nakashima, Yasuhiro van Handel, Ben Sasidharan, Rajkumar Harmon, Andrew W. Shin, Jae-Ho Schwartz, Robert J. Conway, Simon J. Harvey, Richard P. Pashmforoush, Mohammad Mikkola, Hanna K. A. Nakano, Atsushi |
author_sort | Nakano, Haruko |
collection | PubMed |
description | Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a hemogenic organ akin to the dorsal aorta. Here we examine the hemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Hemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the hemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells expressing cardiac markers serve as a de novo source for transient definitive hematopoietic progenitors. |
format | Online Article Text |
id | pubmed-3612528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-36125282013-09-05 Hemogenic endocardium contributes to transient definitive hematopoiesis Nakano, Haruko Nakano, Haruko Liu, Xiaoqian Arshi, Armin Nakashima, Yasuhiro van Handel, Ben Sasidharan, Rajkumar Harmon, Andrew W. Shin, Jae-Ho Schwartz, Robert J. Conway, Simon J. Harvey, Richard P. Pashmforoush, Mohammad Mikkola, Hanna K. A. Nakano, Atsushi Nat Commun Article Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a hemogenic organ akin to the dorsal aorta. Here we examine the hemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Hemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the hemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells expressing cardiac markers serve as a de novo source for transient definitive hematopoietic progenitors. 2013 /pmc/articles/PMC3612528/ /pubmed/23463007 http://dx.doi.org/10.1038/ncomms2569 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Nakano, Haruko Nakano, Haruko Liu, Xiaoqian Arshi, Armin Nakashima, Yasuhiro van Handel, Ben Sasidharan, Rajkumar Harmon, Andrew W. Shin, Jae-Ho Schwartz, Robert J. Conway, Simon J. Harvey, Richard P. Pashmforoush, Mohammad Mikkola, Hanna K. A. Nakano, Atsushi Hemogenic endocardium contributes to transient definitive hematopoiesis |
title | Hemogenic endocardium contributes to transient definitive hematopoiesis |
title_full | Hemogenic endocardium contributes to transient definitive hematopoiesis |
title_fullStr | Hemogenic endocardium contributes to transient definitive hematopoiesis |
title_full_unstemmed | Hemogenic endocardium contributes to transient definitive hematopoiesis |
title_short | Hemogenic endocardium contributes to transient definitive hematopoiesis |
title_sort | hemogenic endocardium contributes to transient definitive hematopoiesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612528/ https://www.ncbi.nlm.nih.gov/pubmed/23463007 http://dx.doi.org/10.1038/ncomms2569 |
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