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In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas

Interactions between distinct motor cortical areas are essential for coordinated motor behaviors. In rodents, the motor cortical forelimb areas are divided into at least two distinct areas: the rostral forelimb area (RFA) and the caudal forelimb area (CFA). The RFA is thought to be an equivalent of...

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Autores principales: Hira, Riichiro, Ohkubo, Fuki, Tanaka, Yasuhiro R., Masamizu, Yoshito, Augustine, George J., Kasai, Haruo, Matsuzaki, Masanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612597/
https://www.ncbi.nlm.nih.gov/pubmed/23554588
http://dx.doi.org/10.3389/fncir.2013.00055
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author Hira, Riichiro
Ohkubo, Fuki
Tanaka, Yasuhiro R.
Masamizu, Yoshito
Augustine, George J.
Kasai, Haruo
Matsuzaki, Masanori
author_facet Hira, Riichiro
Ohkubo, Fuki
Tanaka, Yasuhiro R.
Masamizu, Yoshito
Augustine, George J.
Kasai, Haruo
Matsuzaki, Masanori
author_sort Hira, Riichiro
collection PubMed
description Interactions between distinct motor cortical areas are essential for coordinated motor behaviors. In rodents, the motor cortical forelimb areas are divided into at least two distinct areas: the rostral forelimb area (RFA) and the caudal forelimb area (CFA). The RFA is thought to be an equivalent of the premotor cortex (PM) in primates, whereas the CFA is believed to be an equivalent of the primary motor cortex. Although reciprocal connections between the RFA and the CFA have been anatomically identified in rats, it is unknown whether there are functional connections between these areas that can induce postsynaptic spikes. In this study, we used an in vivo Channelrhodopsin-2 (ChR2) photostimulation method to trace the functional connections between the mouse RFA and CFA. Simultaneous electrical recordings were utilized to detect spiking activities induced by synaptic inputs originating from photostimulated areas. This method, in combination with anatomical tracing, demonstrated that the RFA receives strong functional projections from layer 2/3 and/or layer 5a, but not from layer 5b (L5b), of the CFA. Further, the CFA receives strong projections from L5b neurons of the RFA. The onset latency of electrical responses evoked in remote areas upon photostimulation of the other areas was approximately 10 ms, which is consistent with the synaptic connectivity between these areas. Our results suggest that neuronal activities in the RFA and the CFA during movements are formed through asymmetric reciprocal connections.
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spelling pubmed-36125972013-04-01 In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas Hira, Riichiro Ohkubo, Fuki Tanaka, Yasuhiro R. Masamizu, Yoshito Augustine, George J. Kasai, Haruo Matsuzaki, Masanori Front Neural Circuits Neuroscience Interactions between distinct motor cortical areas are essential for coordinated motor behaviors. In rodents, the motor cortical forelimb areas are divided into at least two distinct areas: the rostral forelimb area (RFA) and the caudal forelimb area (CFA). The RFA is thought to be an equivalent of the premotor cortex (PM) in primates, whereas the CFA is believed to be an equivalent of the primary motor cortex. Although reciprocal connections between the RFA and the CFA have been anatomically identified in rats, it is unknown whether there are functional connections between these areas that can induce postsynaptic spikes. In this study, we used an in vivo Channelrhodopsin-2 (ChR2) photostimulation method to trace the functional connections between the mouse RFA and CFA. Simultaneous electrical recordings were utilized to detect spiking activities induced by synaptic inputs originating from photostimulated areas. This method, in combination with anatomical tracing, demonstrated that the RFA receives strong functional projections from layer 2/3 and/or layer 5a, but not from layer 5b (L5b), of the CFA. Further, the CFA receives strong projections from L5b neurons of the RFA. The onset latency of electrical responses evoked in remote areas upon photostimulation of the other areas was approximately 10 ms, which is consistent with the synaptic connectivity between these areas. Our results suggest that neuronal activities in the RFA and the CFA during movements are formed through asymmetric reciprocal connections. Frontiers Media S.A. 2013-04-01 /pmc/articles/PMC3612597/ /pubmed/23554588 http://dx.doi.org/10.3389/fncir.2013.00055 Text en Copyright © 2013 Hira, Ohkubo, Tanaka, Masamizu, Augustine, Kasai and Matsuzaki. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Neuroscience
Hira, Riichiro
Ohkubo, Fuki
Tanaka, Yasuhiro R.
Masamizu, Yoshito
Augustine, George J.
Kasai, Haruo
Matsuzaki, Masanori
In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title_full In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title_fullStr In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title_full_unstemmed In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title_short In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
title_sort in vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612597/
https://www.ncbi.nlm.nih.gov/pubmed/23554588
http://dx.doi.org/10.3389/fncir.2013.00055
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