Recombinant Human Butyrylcholinesterase As a New-Age Bioscavenger Drug: Development of the Expression System

Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encod...

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Detalles Bibliográficos
Autores principales: Ilyushin, D.G., Haertley, O.M., Bobik, T.V., Shamborant, O.G., Surina, E.A., Knorre, V.D., Masson, P., Smirnov, I.V., Gabibov, A.G., Ponomarenko, N.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612827/
https://www.ncbi.nlm.nih.gov/pubmed/23556132
Descripción
Sumario:Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.

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