Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells

Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-indep...

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Autores principales: Shevchuk, Z., Yurchenko, M. Y., Darekar, S. D., Holodnuka-Kholodnyuk, I., Kashuba, V. I., Kashuba, E. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612828/
https://www.ncbi.nlm.nih.gov/pubmed/23556133
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author Shevchuk, Z.
Yurchenko, M. Y.
Darekar, S. D.
Holodnuka-Kholodnyuk, I.
Kashuba, V. I.
Kashuba, E. V.
author_facet Shevchuk, Z.
Yurchenko, M. Y.
Darekar, S. D.
Holodnuka-Kholodnyuk, I.
Kashuba, V. I.
Kashuba, E. V.
author_sort Shevchuk, Z.
collection PubMed
description Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones.
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spelling pubmed-36128282013-04-03 Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells Shevchuk, Z. Yurchenko, M. Y. Darekar, S. D. Holodnuka-Kholodnyuk, I. Kashuba, V. I. Kashuba, E. V. Acta Naturae Research Article Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones. A.I. Gordeyev 2013 /pmc/articles/PMC3612828/ /pubmed/23556133 Text en Copyright © 2013 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shevchuk, Z.
Yurchenko, M. Y.
Darekar, S. D.
Holodnuka-Kholodnyuk, I.
Kashuba, V. I.
Kashuba, E. V.
Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title_full Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title_fullStr Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title_full_unstemmed Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title_short Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
title_sort overexpression of mrps18-2 in cancer cell lines results in appearance of multinucleated cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612828/
https://www.ncbi.nlm.nih.gov/pubmed/23556133
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