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Expression Systems and Species Used for Transgenic Animal Bioreactors

Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were...

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Detalles Bibliográficos
Autores principales: Wang, Yanli, Zhao, Sihai, Bai, Liang, Fan, Jianglin, Liu, Enqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613084/
https://www.ncbi.nlm.nih.gov/pubmed/23586046
http://dx.doi.org/10.1155/2013/580463
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author Wang, Yanli
Zhao, Sihai
Bai, Liang
Fan, Jianglin
Liu, Enqi
author_facet Wang, Yanli
Zhao, Sihai
Bai, Liang
Fan, Jianglin
Liu, Enqi
author_sort Wang, Yanli
collection PubMed
description Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were potentially ideal bioreactors for the synthesis of pharmaceutical protein complexes. Compared with other transgenic animal expression systems (egg white, blood, urine, seminal plasma, and silkworm cocoon), the mammary glands of transgenic animals have enormous potential. Compared with other mammalian species (pig, goat, sheep, and cow) that are currently being studied as bioreactors, rabbits offer many advantages: high fertility, easy generation of transgenic founders and offspring, insensitivity to prion diseases, relatively high milk production, and no transmission of severe diseases to humans. Noticeably, for a small- or medium-sized facility, the rabbit system is ideal to produce up to 50 kg of protein per year, considering both economical and hygienic aspects; rabbits are attractive candidates for the mammary-gland-specific expression of recombinant proteins. We also reviewed recombinant proteins that have been produced by targeted expression in the mammary glands of rabbits and discussed the limitations of transgenic animal bioreactors.
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spelling pubmed-36130842013-04-12 Expression Systems and Species Used for Transgenic Animal Bioreactors Wang, Yanli Zhao, Sihai Bai, Liang Fan, Jianglin Liu, Enqi Biomed Res Int Review Article Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were potentially ideal bioreactors for the synthesis of pharmaceutical protein complexes. Compared with other transgenic animal expression systems (egg white, blood, urine, seminal plasma, and silkworm cocoon), the mammary glands of transgenic animals have enormous potential. Compared with other mammalian species (pig, goat, sheep, and cow) that are currently being studied as bioreactors, rabbits offer many advantages: high fertility, easy generation of transgenic founders and offspring, insensitivity to prion diseases, relatively high milk production, and no transmission of severe diseases to humans. Noticeably, for a small- or medium-sized facility, the rabbit system is ideal to produce up to 50 kg of protein per year, considering both economical and hygienic aspects; rabbits are attractive candidates for the mammary-gland-specific expression of recombinant proteins. We also reviewed recombinant proteins that have been produced by targeted expression in the mammary glands of rabbits and discussed the limitations of transgenic animal bioreactors. Hindawi Publishing Corporation 2013 2013-03-17 /pmc/articles/PMC3613084/ /pubmed/23586046 http://dx.doi.org/10.1155/2013/580463 Text en Copyright © 2013 Yanli Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Wang, Yanli
Zhao, Sihai
Bai, Liang
Fan, Jianglin
Liu, Enqi
Expression Systems and Species Used for Transgenic Animal Bioreactors
title Expression Systems and Species Used for Transgenic Animal Bioreactors
title_full Expression Systems and Species Used for Transgenic Animal Bioreactors
title_fullStr Expression Systems and Species Used for Transgenic Animal Bioreactors
title_full_unstemmed Expression Systems and Species Used for Transgenic Animal Bioreactors
title_short Expression Systems and Species Used for Transgenic Animal Bioreactors
title_sort expression systems and species used for transgenic animal bioreactors
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613084/
https://www.ncbi.nlm.nih.gov/pubmed/23586046
http://dx.doi.org/10.1155/2013/580463
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