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Expression Systems and Species Used for Transgenic Animal Bioreactors
Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613084/ https://www.ncbi.nlm.nih.gov/pubmed/23586046 http://dx.doi.org/10.1155/2013/580463 |
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author | Wang, Yanli Zhao, Sihai Bai, Liang Fan, Jianglin Liu, Enqi |
author_facet | Wang, Yanli Zhao, Sihai Bai, Liang Fan, Jianglin Liu, Enqi |
author_sort | Wang, Yanli |
collection | PubMed |
description | Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were potentially ideal bioreactors for the synthesis of pharmaceutical protein complexes. Compared with other transgenic animal expression systems (egg white, blood, urine, seminal plasma, and silkworm cocoon), the mammary glands of transgenic animals have enormous potential. Compared with other mammalian species (pig, goat, sheep, and cow) that are currently being studied as bioreactors, rabbits offer many advantages: high fertility, easy generation of transgenic founders and offspring, insensitivity to prion diseases, relatively high milk production, and no transmission of severe diseases to humans. Noticeably, for a small- or medium-sized facility, the rabbit system is ideal to produce up to 50 kg of protein per year, considering both economical and hygienic aspects; rabbits are attractive candidates for the mammary-gland-specific expression of recombinant proteins. We also reviewed recombinant proteins that have been produced by targeted expression in the mammary glands of rabbits and discussed the limitations of transgenic animal bioreactors. |
format | Online Article Text |
id | pubmed-3613084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36130842013-04-12 Expression Systems and Species Used for Transgenic Animal Bioreactors Wang, Yanli Zhao, Sihai Bai, Liang Fan, Jianglin Liu, Enqi Biomed Res Int Review Article Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were potentially ideal bioreactors for the synthesis of pharmaceutical protein complexes. Compared with other transgenic animal expression systems (egg white, blood, urine, seminal plasma, and silkworm cocoon), the mammary glands of transgenic animals have enormous potential. Compared with other mammalian species (pig, goat, sheep, and cow) that are currently being studied as bioreactors, rabbits offer many advantages: high fertility, easy generation of transgenic founders and offspring, insensitivity to prion diseases, relatively high milk production, and no transmission of severe diseases to humans. Noticeably, for a small- or medium-sized facility, the rabbit system is ideal to produce up to 50 kg of protein per year, considering both economical and hygienic aspects; rabbits are attractive candidates for the mammary-gland-specific expression of recombinant proteins. We also reviewed recombinant proteins that have been produced by targeted expression in the mammary glands of rabbits and discussed the limitations of transgenic animal bioreactors. Hindawi Publishing Corporation 2013 2013-03-17 /pmc/articles/PMC3613084/ /pubmed/23586046 http://dx.doi.org/10.1155/2013/580463 Text en Copyright © 2013 Yanli Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Wang, Yanli Zhao, Sihai Bai, Liang Fan, Jianglin Liu, Enqi Expression Systems and Species Used for Transgenic Animal Bioreactors |
title | Expression Systems and Species Used for Transgenic Animal Bioreactors |
title_full | Expression Systems and Species Used for Transgenic Animal Bioreactors |
title_fullStr | Expression Systems and Species Used for Transgenic Animal Bioreactors |
title_full_unstemmed | Expression Systems and Species Used for Transgenic Animal Bioreactors |
title_short | Expression Systems and Species Used for Transgenic Animal Bioreactors |
title_sort | expression systems and species used for transgenic animal bioreactors |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613084/ https://www.ncbi.nlm.nih.gov/pubmed/23586046 http://dx.doi.org/10.1155/2013/580463 |
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