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Aldose Reductase Inhibitory Activity of Compounds from Zea mays L.
Aldose reductase (AR) inhibitors have a considerable therapeutic potential against diabetes complications and do not increase the risk of hypoglycemia. Through bioassay-guided fractionation of an EtOH extract of the kernel from purple corn (Zea mays L.), 7 nonanthocyanin phenolic compounds (compound...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613087/ https://www.ncbi.nlm.nih.gov/pubmed/23586057 http://dx.doi.org/10.1155/2013/727143 |
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author | Kim, Tae Hyeon Kim, Jin Kyu Kang, Young-Hee Lee, Jae-Yong Kang, Il Jun Lim, Soon Sung |
author_facet | Kim, Tae Hyeon Kim, Jin Kyu Kang, Young-Hee Lee, Jae-Yong Kang, Il Jun Lim, Soon Sung |
author_sort | Kim, Tae Hyeon |
collection | PubMed |
description | Aldose reductase (AR) inhibitors have a considerable therapeutic potential against diabetes complications and do not increase the risk of hypoglycemia. Through bioassay-guided fractionation of an EtOH extract of the kernel from purple corn (Zea mays L.), 7 nonanthocyanin phenolic compounds (compound 1–7) and 5 anthocyanins (compound 8–12) were isolated. These compounds were investigated by rat lens aldose reductase (RLAR) inhibitory assays. Kinetic analyses of recombinant human aldose reductase (rhAR) were performed, and intracellular galactitol levels were measured. Hirsutrin, one of 12 isolated compounds, showed the most potent RLAR inhibitory activity (IC(50), 4.78 μM). In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/substrate concentration, hirsutrin showed competitive inhibition against rhAR. Furthermore, hirsutrin inhibited galactitol formation in rat lens and erythrocytes sample incubated with a high concentration of galactose; this finding indicates that hirsutrin may effectively prevent osmotic stress in hyperglycemia. Therefore, hirsutrin derived from Zea mays L. may be a potential therapeutic agent against diabetes complications. |
format | Online Article Text |
id | pubmed-3613087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36130872013-04-12 Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. Kim, Tae Hyeon Kim, Jin Kyu Kang, Young-Hee Lee, Jae-Yong Kang, Il Jun Lim, Soon Sung Biomed Res Int Research Article Aldose reductase (AR) inhibitors have a considerable therapeutic potential against diabetes complications and do not increase the risk of hypoglycemia. Through bioassay-guided fractionation of an EtOH extract of the kernel from purple corn (Zea mays L.), 7 nonanthocyanin phenolic compounds (compound 1–7) and 5 anthocyanins (compound 8–12) were isolated. These compounds were investigated by rat lens aldose reductase (RLAR) inhibitory assays. Kinetic analyses of recombinant human aldose reductase (rhAR) were performed, and intracellular galactitol levels were measured. Hirsutrin, one of 12 isolated compounds, showed the most potent RLAR inhibitory activity (IC(50), 4.78 μM). In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/substrate concentration, hirsutrin showed competitive inhibition against rhAR. Furthermore, hirsutrin inhibited galactitol formation in rat lens and erythrocytes sample incubated with a high concentration of galactose; this finding indicates that hirsutrin may effectively prevent osmotic stress in hyperglycemia. Therefore, hirsutrin derived from Zea mays L. may be a potential therapeutic agent against diabetes complications. Hindawi Publishing Corporation 2013 2013-03-17 /pmc/articles/PMC3613087/ /pubmed/23586057 http://dx.doi.org/10.1155/2013/727143 Text en Copyright © 2013 Tae Hyeon Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kim, Tae Hyeon Kim, Jin Kyu Kang, Young-Hee Lee, Jae-Yong Kang, Il Jun Lim, Soon Sung Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title | Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title_full | Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title_fullStr | Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title_full_unstemmed | Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title_short | Aldose Reductase Inhibitory Activity of Compounds from Zea mays L. |
title_sort | aldose reductase inhibitory activity of compounds from zea mays l. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613087/ https://www.ncbi.nlm.nih.gov/pubmed/23586057 http://dx.doi.org/10.1155/2013/727143 |
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