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The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal

Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the sur...

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Autores principales: Zakharova, Yulia R., Galachyants, Yuri P., Kurilkina, Maria I., Likhoshvay, Alexander V., Petrova, Darya P., Shishlyannikov, Sergey M., Ravin, Nikolai V., Mardanov, Andrey V., Beletsky, Alexey V., Likhoshway, Yelena V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613400/
https://www.ncbi.nlm.nih.gov/pubmed/23560063
http://dx.doi.org/10.1371/journal.pone.0059977
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author Zakharova, Yulia R.
Galachyants, Yuri P.
Kurilkina, Maria I.
Likhoshvay, Alexander V.
Petrova, Darya P.
Shishlyannikov, Sergey M.
Ravin, Nikolai V.
Mardanov, Andrey V.
Beletsky, Alexey V.
Likhoshway, Yelena V.
author_facet Zakharova, Yulia R.
Galachyants, Yuri P.
Kurilkina, Maria I.
Likhoshvay, Alexander V.
Petrova, Darya P.
Shishlyannikov, Sergey M.
Ravin, Nikolai V.
Mardanov, Andrey V.
Beletsky, Alexey V.
Likhoshway, Yelena V.
author_sort Zakharova, Yulia R.
collection PubMed
description Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the surface layer of bottom sediments decrease with depth; in the near-bottom water layer, the majority of bacteria are associated with diatom cells, being located either on the cell surface or within the cell. The structure of microbial community in the near-bottom water layer has been characterized by pyrosequencing of the 16S rRNA gene, which has revealed 149 208 unique sequences. According to the results of metagenomic analysis, the community is dominated by representatives of Proteobacteria (41.9%), Actinobacteria (16%); then follow Acidobacteria (6.9%), Cyanobacteria (5%), Bacteroidetes (4.7%), Firmicutes (2.8%), Nitrospira (1.6%), and Verrucomicrobia (1%); other phylotypes account for less than 1% each. For 18.7% of the sequences, taxonomic identification has been possible only to the Bacteria domain level. Many bacteria identified to the genus level have close relatives occurring in other aquatic ecosystems and soils. The metagenome of the bacterial community from the near-bottom water layer also contains 16S rRNA gene sequences found in previously isolated bacterial strains possessing hydrolytic enzyme activity. These data show that potential degraders of diatoms occur among the vast variety of microorganisms in the near-bottom water of Lake Baikal.
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spelling pubmed-36134002013-04-04 The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal Zakharova, Yulia R. Galachyants, Yuri P. Kurilkina, Maria I. Likhoshvay, Alexander V. Petrova, Darya P. Shishlyannikov, Sergey M. Ravin, Nikolai V. Mardanov, Andrey V. Beletsky, Alexey V. Likhoshway, Yelena V. PLoS One Research Article Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the surface layer of bottom sediments decrease with depth; in the near-bottom water layer, the majority of bacteria are associated with diatom cells, being located either on the cell surface or within the cell. The structure of microbial community in the near-bottom water layer has been characterized by pyrosequencing of the 16S rRNA gene, which has revealed 149 208 unique sequences. According to the results of metagenomic analysis, the community is dominated by representatives of Proteobacteria (41.9%), Actinobacteria (16%); then follow Acidobacteria (6.9%), Cyanobacteria (5%), Bacteroidetes (4.7%), Firmicutes (2.8%), Nitrospira (1.6%), and Verrucomicrobia (1%); other phylotypes account for less than 1% each. For 18.7% of the sequences, taxonomic identification has been possible only to the Bacteria domain level. Many bacteria identified to the genus level have close relatives occurring in other aquatic ecosystems and soils. The metagenome of the bacterial community from the near-bottom water layer also contains 16S rRNA gene sequences found in previously isolated bacterial strains possessing hydrolytic enzyme activity. These data show that potential degraders of diatoms occur among the vast variety of microorganisms in the near-bottom water of Lake Baikal. Public Library of Science 2013-04-01 /pmc/articles/PMC3613400/ /pubmed/23560063 http://dx.doi.org/10.1371/journal.pone.0059977 Text en © 2013 Zakharova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zakharova, Yulia R.
Galachyants, Yuri P.
Kurilkina, Maria I.
Likhoshvay, Alexander V.
Petrova, Darya P.
Shishlyannikov, Sergey M.
Ravin, Nikolai V.
Mardanov, Andrey V.
Beletsky, Alexey V.
Likhoshway, Yelena V.
The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title_full The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title_fullStr The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title_full_unstemmed The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title_short The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal
title_sort structure of microbial community and degradation of diatoms in the deep near-bottom layer of lake baikal
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613400/
https://www.ncbi.nlm.nih.gov/pubmed/23560063
http://dx.doi.org/10.1371/journal.pone.0059977
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