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Binding to F-actin guides cadherin cluster assembly, stability, and movement

The cadherin extracellular region produces intercellular adhesion clusters through trans- and cis-intercadherin bonds, and the intracellular region connects these clusters to the cytoskeleton. To elucidate the interdependence of these binding events, cadherin adhesion was reconstructed from the mini...

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Detalles Bibliográficos
Autores principales: Hong, Soonjin, Troyanovsky, Regina B., Troyanovsky, Sergey M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613698/
https://www.ncbi.nlm.nih.gov/pubmed/23547031
http://dx.doi.org/10.1083/jcb.201211054
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author Hong, Soonjin
Troyanovsky, Regina B.
Troyanovsky, Sergey M.
author_facet Hong, Soonjin
Troyanovsky, Regina B.
Troyanovsky, Sergey M.
author_sort Hong, Soonjin
collection PubMed
description The cadherin extracellular region produces intercellular adhesion clusters through trans- and cis-intercadherin bonds, and the intracellular region connects these clusters to the cytoskeleton. To elucidate the interdependence of these binding events, cadherin adhesion was reconstructed from the minimal number of structural elements. F-actin–uncoupled adhesive clusters displayed high instability and random motion. Their assembly required a cadherin cis-binding interface. Coupling these clusters with F-actin through an α-catenin actin-binding domain (αABD) dramatically extended cluster lifetime and conferred direction to cluster motility. In addition, αABD partially lifted the requirement for the cis-interface for cluster assembly. Even more dramatic enhancement of cadherin clustering was observed if αABD was joined with cadherin through a flexible linker or if it was replaced with an actin-binding domain of utrophin. These data present direct evidence that binding to F-actin stabilizes cadherin clusters and cooperates with the cis-interface in cadherin clustering. Such cooperation apparently synchronizes extracellular and intracellular binding events in the process of adherens junction assembly.
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spelling pubmed-36136982013-10-01 Binding to F-actin guides cadherin cluster assembly, stability, and movement Hong, Soonjin Troyanovsky, Regina B. Troyanovsky, Sergey M. J Cell Biol Research Articles The cadherin extracellular region produces intercellular adhesion clusters through trans- and cis-intercadherin bonds, and the intracellular region connects these clusters to the cytoskeleton. To elucidate the interdependence of these binding events, cadherin adhesion was reconstructed from the minimal number of structural elements. F-actin–uncoupled adhesive clusters displayed high instability and random motion. Their assembly required a cadherin cis-binding interface. Coupling these clusters with F-actin through an α-catenin actin-binding domain (αABD) dramatically extended cluster lifetime and conferred direction to cluster motility. In addition, αABD partially lifted the requirement for the cis-interface for cluster assembly. Even more dramatic enhancement of cadherin clustering was observed if αABD was joined with cadherin through a flexible linker or if it was replaced with an actin-binding domain of utrophin. These data present direct evidence that binding to F-actin stabilizes cadherin clusters and cooperates with the cis-interface in cadherin clustering. Such cooperation apparently synchronizes extracellular and intracellular binding events in the process of adherens junction assembly. The Rockefeller University Press 2013-04-01 /pmc/articles/PMC3613698/ /pubmed/23547031 http://dx.doi.org/10.1083/jcb.201211054 Text en © 2013 Hong et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Hong, Soonjin
Troyanovsky, Regina B.
Troyanovsky, Sergey M.
Binding to F-actin guides cadherin cluster assembly, stability, and movement
title Binding to F-actin guides cadherin cluster assembly, stability, and movement
title_full Binding to F-actin guides cadherin cluster assembly, stability, and movement
title_fullStr Binding to F-actin guides cadherin cluster assembly, stability, and movement
title_full_unstemmed Binding to F-actin guides cadherin cluster assembly, stability, and movement
title_short Binding to F-actin guides cadherin cluster assembly, stability, and movement
title_sort binding to f-actin guides cadherin cluster assembly, stability, and movement
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613698/
https://www.ncbi.nlm.nih.gov/pubmed/23547031
http://dx.doi.org/10.1083/jcb.201211054
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