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Improved use of a public good selects for the evolution of undifferentiated multicellularity
We do not know how or why multicellularity evolved. We used the budding yeast, Saccharomyces cerevisiae, to ask whether nutrients that must be digested extracellularly select for the evolution of undifferentiated multicellularity. Because yeast use invertase to hydrolyze sucrose extracellularly and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614033/ https://www.ncbi.nlm.nih.gov/pubmed/23577233 http://dx.doi.org/10.7554/eLife.00367 |
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author | Koschwanez, John H Foster, Kevin R Murray, Andrew W |
author_facet | Koschwanez, John H Foster, Kevin R Murray, Andrew W |
author_sort | Koschwanez, John H |
collection | PubMed |
description | We do not know how or why multicellularity evolved. We used the budding yeast, Saccharomyces cerevisiae, to ask whether nutrients that must be digested extracellularly select for the evolution of undifferentiated multicellularity. Because yeast use invertase to hydrolyze sucrose extracellularly and import the resulting monosaccharides, single cells cannot grow at low cell and sucrose concentrations. Three engineered strategies overcame this problem: forming multicellular clumps, importing sucrose before hydrolysis, and increasing invertase expression. We evolved populations in low sucrose to ask which strategy they would adopt. Of 12 successful clones, 11 formed multicellular clumps through incomplete cell separation, 10 increased invertase expression, none imported sucrose, and 11 increased hexose transporter expression, a strategy we had not engineered. Identifying causal mutations revealed genes and pathways, which frequently contributed to the evolved phenotype. Our study shows that combining rational design with experimental evolution can help evaluate hypotheses about evolutionary strategies. DOI: http://dx.doi.org/10.7554/eLife.00367.001 |
format | Online Article Text |
id | pubmed-3614033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-36140332013-04-10 Improved use of a public good selects for the evolution of undifferentiated multicellularity Koschwanez, John H Foster, Kevin R Murray, Andrew W eLife Genomics and Evolutionary Biology We do not know how or why multicellularity evolved. We used the budding yeast, Saccharomyces cerevisiae, to ask whether nutrients that must be digested extracellularly select for the evolution of undifferentiated multicellularity. Because yeast use invertase to hydrolyze sucrose extracellularly and import the resulting monosaccharides, single cells cannot grow at low cell and sucrose concentrations. Three engineered strategies overcame this problem: forming multicellular clumps, importing sucrose before hydrolysis, and increasing invertase expression. We evolved populations in low sucrose to ask which strategy they would adopt. Of 12 successful clones, 11 formed multicellular clumps through incomplete cell separation, 10 increased invertase expression, none imported sucrose, and 11 increased hexose transporter expression, a strategy we had not engineered. Identifying causal mutations revealed genes and pathways, which frequently contributed to the evolved phenotype. Our study shows that combining rational design with experimental evolution can help evaluate hypotheses about evolutionary strategies. DOI: http://dx.doi.org/10.7554/eLife.00367.001 eLife Sciences Publications, Ltd 2013-04-02 /pmc/articles/PMC3614033/ /pubmed/23577233 http://dx.doi.org/10.7554/eLife.00367 Text en Copyright © 2013, Koschwanez et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Genomics and Evolutionary Biology Koschwanez, John H Foster, Kevin R Murray, Andrew W Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title | Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title_full | Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title_fullStr | Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title_full_unstemmed | Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title_short | Improved use of a public good selects for the evolution of undifferentiated multicellularity |
title_sort | improved use of a public good selects for the evolution of undifferentiated multicellularity |
topic | Genomics and Evolutionary Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614033/ https://www.ncbi.nlm.nih.gov/pubmed/23577233 http://dx.doi.org/10.7554/eLife.00367 |
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