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Sponge-Like: A New Protocol for Preparing Bacterial Ghosts

Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications. In this study, for the first time we introduce a new protocol for BGs production. E. coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general pr...

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Detalles Bibliográficos
Autores principales: Amara, Amro A., Salem-Bekhit, Mounir M., Alanazi, Fars K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614036/
https://www.ncbi.nlm.nih.gov/pubmed/23576904
http://dx.doi.org/10.1155/2013/545741
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author Amara, Amro A.
Salem-Bekhit, Mounir M.
Alanazi, Fars K.
author_facet Amara, Amro A.
Salem-Bekhit, Mounir M.
Alanazi, Fars K.
author_sort Amara, Amro A.
collection PubMed
description Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications. In this study, for the first time we introduce a new protocol for BGs production. E. coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general protocol for BGs preparation. The protocol is based on using active chemical compounds in concentrations less than the Minimum Inhibition Concentration (MIC). Those chemical compounds are SDS, NaOH, and H(2)O(2). Plackett-Burman experimental design was used to map the best conditions for BGs production. Normal and electronic microscopes were used to evaluate the BGs quality (BGQ). Spectrophotometer was used to evaluate the amount of the released protein and DNA. Agarose gel electrophoresis was used to determine the existence of any residue of DNA after each BGs preparation. Viable cells, which existed after running this protocol, were subjected to lysis by inducing the lysozyme gene carried on pLysS plasmid. This protocol is able to produce BGs that can be used in different biotechnological applications.
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spelling pubmed-36140362013-04-10 Sponge-Like: A New Protocol for Preparing Bacterial Ghosts Amara, Amro A. Salem-Bekhit, Mounir M. Alanazi, Fars K. ScientificWorldJournal Research Article Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications. In this study, for the first time we introduce a new protocol for BGs production. E. coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general protocol for BGs preparation. The protocol is based on using active chemical compounds in concentrations less than the Minimum Inhibition Concentration (MIC). Those chemical compounds are SDS, NaOH, and H(2)O(2). Plackett-Burman experimental design was used to map the best conditions for BGs production. Normal and electronic microscopes were used to evaluate the BGs quality (BGQ). Spectrophotometer was used to evaluate the amount of the released protein and DNA. Agarose gel electrophoresis was used to determine the existence of any residue of DNA after each BGs preparation. Viable cells, which existed after running this protocol, were subjected to lysis by inducing the lysozyme gene carried on pLysS plasmid. This protocol is able to produce BGs that can be used in different biotechnological applications. Hindawi Publishing Corporation 2013-03-18 /pmc/articles/PMC3614036/ /pubmed/23576904 http://dx.doi.org/10.1155/2013/545741 Text en Copyright © 2013 Amro A. Amara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Amara, Amro A.
Salem-Bekhit, Mounir M.
Alanazi, Fars K.
Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title_full Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title_fullStr Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title_full_unstemmed Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title_short Sponge-Like: A New Protocol for Preparing Bacterial Ghosts
title_sort sponge-like: a new protocol for preparing bacterial ghosts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614036/
https://www.ncbi.nlm.nih.gov/pubmed/23576904
http://dx.doi.org/10.1155/2013/545741
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