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Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary

Angiotensin II increases blood pressure and stimulates thirst and sodium appetite in the brain. It also stimulates secretion of aldosterone from the adrenal zona glomerulosa and epinephrine from the adrenal medulla. The rat has 3 subtypes of angiotensin II receptors: AT(1a), AT(1b), and AT(2). mRNAs...

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Autores principales: Premer, Courtney, Lamondin, Courtney, Mitzey, Ann, Speth, Robert C., Brownfield, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614054/
https://www.ncbi.nlm.nih.gov/pubmed/23573410
http://dx.doi.org/10.1155/2013/175428
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author Premer, Courtney
Lamondin, Courtney
Mitzey, Ann
Speth, Robert C.
Brownfield, Mark S.
author_facet Premer, Courtney
Lamondin, Courtney
Mitzey, Ann
Speth, Robert C.
Brownfield, Mark S.
author_sort Premer, Courtney
collection PubMed
description Angiotensin II increases blood pressure and stimulates thirst and sodium appetite in the brain. It also stimulates secretion of aldosterone from the adrenal zona glomerulosa and epinephrine from the adrenal medulla. The rat has 3 subtypes of angiotensin II receptors: AT(1a), AT(1b), and AT(2). mRNAs for all three subtypes occur in the adrenal and brain. To immunohistochemically differentiate these receptor subtypes, rabbits were immunized with C-terminal fragments of these subtypes to generate receptor subtype-specific antibodies. Immunofluorescence revealed AT(1a) and AT(2) receptors in adrenal zona glomerulosa and medulla. AT(1b) immunofluorescence was present in the zona glomerulosa, but not the medulla. Ultrastructural immunogold labeling for the AT(1a) receptor in glomerulosa and medullary cells localized it to plasma membrane, endocytic vesicles, multivesicular bodies, and the nucleus. AT(1b) and AT(2), but not AT(1a), immunofluorescence was observed in the anterior pituitary. Stellate cells were AT(1b) positive while ovoid cells were AT(2) positive. In the brain, neurons were AT(1a), AT(1b), and AT(2) positive, but glia was only AT(1b) positive. Highest levels of AT(1a), AT(1b), and AT(2) receptor immunofluorescence were in the subfornical organ, median eminence, area postrema, paraventricular nucleus, and solitary tract nucleus. These studies complement those employing different techniques to characterize Ang II receptors.
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spelling pubmed-36140542013-04-09 Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary Premer, Courtney Lamondin, Courtney Mitzey, Ann Speth, Robert C. Brownfield, Mark S. Int J Hypertens Research Article Angiotensin II increases blood pressure and stimulates thirst and sodium appetite in the brain. It also stimulates secretion of aldosterone from the adrenal zona glomerulosa and epinephrine from the adrenal medulla. The rat has 3 subtypes of angiotensin II receptors: AT(1a), AT(1b), and AT(2). mRNAs for all three subtypes occur in the adrenal and brain. To immunohistochemically differentiate these receptor subtypes, rabbits were immunized with C-terminal fragments of these subtypes to generate receptor subtype-specific antibodies. Immunofluorescence revealed AT(1a) and AT(2) receptors in adrenal zona glomerulosa and medulla. AT(1b) immunofluorescence was present in the zona glomerulosa, but not the medulla. Ultrastructural immunogold labeling for the AT(1a) receptor in glomerulosa and medullary cells localized it to plasma membrane, endocytic vesicles, multivesicular bodies, and the nucleus. AT(1b) and AT(2), but not AT(1a), immunofluorescence was observed in the anterior pituitary. Stellate cells were AT(1b) positive while ovoid cells were AT(2) positive. In the brain, neurons were AT(1a), AT(1b), and AT(2) positive, but glia was only AT(1b) positive. Highest levels of AT(1a), AT(1b), and AT(2) receptor immunofluorescence were in the subfornical organ, median eminence, area postrema, paraventricular nucleus, and solitary tract nucleus. These studies complement those employing different techniques to characterize Ang II receptors. Hindawi Publishing Corporation 2013 2013-03-19 /pmc/articles/PMC3614054/ /pubmed/23573410 http://dx.doi.org/10.1155/2013/175428 Text en Copyright © 2013 Courtney Premer et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Premer, Courtney
Lamondin, Courtney
Mitzey, Ann
Speth, Robert C.
Brownfield, Mark S.
Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title_full Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title_fullStr Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title_full_unstemmed Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title_short Immunohistochemical Localization of AT(1a), AT(1b), and AT(2) Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary
title_sort immunohistochemical localization of at(1a), at(1b), and at(2) angiotensin ii receptor subtypes in the rat adrenal, pituitary, and brain with a perspective commentary
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614054/
https://www.ncbi.nlm.nih.gov/pubmed/23573410
http://dx.doi.org/10.1155/2013/175428
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