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Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation

Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation induced adsorption, aggregation and adhesion between single- a...

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Autor principal: Süleymanoglu, Erhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Master Publishing Group 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614652/
https://www.ncbi.nlm.nih.gov/pubmed/23675003
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author Süleymanoglu, Erhan
author_facet Süleymanoglu, Erhan
author_sort Süleymanoglu, Erhan
collection PubMed
description Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followed by differential adiabatic scanning microcalorimetry. Nucleic acid condensation and compaction mediated by Mg(2+) was followed, with regard to interfacial interaction with unilamellar vesicles. Microcalorimetric measurements of synthetic phospholipid vesicles and poly(ribo) nucleotides and their ternary complexes with inorganic cations were used to build the thermodynamic model of their structural transitions. The increased thermal stability of the phospholipid bilayers is achieved by affecting their melting transition temperature by nucleic acid induced electrostatic charge screening. Measurements give evidence for the stabilization of polynucleotide helices upon their association with liposomes in presence of divalent metal cations. Such an induced aggregation vesicles either leads to heterogeneous multilamellar DNA-lipid arrangements, or to DNA-induced bilayer destabilization and lipid fusion. The further employment of these polyelectrolyte nanostructures as an improved formulations in therapeutic gene delivery trials, as well as in DNA chromatography is discussed.
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spelling pubmed-36146522013-05-01 Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation Süleymanoglu, Erhan Int J Biomed Sci Article Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followed by differential adiabatic scanning microcalorimetry. Nucleic acid condensation and compaction mediated by Mg(2+) was followed, with regard to interfacial interaction with unilamellar vesicles. Microcalorimetric measurements of synthetic phospholipid vesicles and poly(ribo) nucleotides and their ternary complexes with inorganic cations were used to build the thermodynamic model of their structural transitions. The increased thermal stability of the phospholipid bilayers is achieved by affecting their melting transition temperature by nucleic acid induced electrostatic charge screening. Measurements give evidence for the stabilization of polynucleotide helices upon their association with liposomes in presence of divalent metal cations. Such an induced aggregation vesicles either leads to heterogeneous multilamellar DNA-lipid arrangements, or to DNA-induced bilayer destabilization and lipid fusion. The further employment of these polyelectrolyte nanostructures as an improved formulations in therapeutic gene delivery trials, as well as in DNA chromatography is discussed. Master Publishing Group 2006-12 /pmc/articles/PMC3614652/ /pubmed/23675003 Text en © Erhan Süleymanoglu Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Süleymanoglu, Erhan
Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title_full Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title_fullStr Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title_full_unstemmed Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title_short Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg(2+)-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
title_sort phospholipid-nucleic acid complexation: biomolecular energetics of dna-mg(2+)-phosphatidylcholine ternary complex formation, compaction and relevance as lipoplex formulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614652/
https://www.ncbi.nlm.nih.gov/pubmed/23675003
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