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Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II)
A simple and sensitive spectrophotometric method was developed for the determination of acetazolamide (ACM) in pure form and pharmaceutical preparations. The proposed method is based on the complex formation of acetazolamide with Palladium (II) chloride in acetate buffer pH5.4 and measuring the abso...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Master Publishing Group
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614737/ https://www.ncbi.nlm.nih.gov/pubmed/23675188 |
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author | Walash, M. I. El-Brashy, A. El-Enany, N. Wahba, M. E. K. |
author_facet | Walash, M. I. El-Brashy, A. El-Enany, N. Wahba, M. E. K. |
author_sort | Walash, M. I. |
collection | PubMed |
description | A simple and sensitive spectrophotometric method was developed for the determination of acetazolamide (ACM) in pure form and pharmaceutical preparations. The proposed method is based on the complex formation of acetazolamide with Palladium (II) chloride in acetate buffer pH5.4 and measuring the absorbance at 308 nm. The absorbance- concentration plot was rectilinear over the concentration range of 5-70 μg/ml with a minimum detection limit (LOD) of 0.98 μg/ml, limit of quantification (LOQ) of 2.96 μg/ml, and a molar absorptivity ζ=2.7 × 10(3) L/mol.cm. The factors affecting the absorbance of the formed complex were carefully studied and optimized. The composition of the complex as well as its stability constant was also investigated. The proposed method was applied for the determination of acetazolamide in its tablets and the results obtained were favorably compared with those obtained using the official method. A proposal of the reaction pathway was postulated. |
format | Online Article Text |
id | pubmed-3614737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Master Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36147372013-05-01 Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) Walash, M. I. El-Brashy, A. El-Enany, N. Wahba, M. E. K. Int J Biomed Sci Article A simple and sensitive spectrophotometric method was developed for the determination of acetazolamide (ACM) in pure form and pharmaceutical preparations. The proposed method is based on the complex formation of acetazolamide with Palladium (II) chloride in acetate buffer pH5.4 and measuring the absorbance at 308 nm. The absorbance- concentration plot was rectilinear over the concentration range of 5-70 μg/ml with a minimum detection limit (LOD) of 0.98 μg/ml, limit of quantification (LOQ) of 2.96 μg/ml, and a molar absorptivity ζ=2.7 × 10(3) L/mol.cm. The factors affecting the absorbance of the formed complex were carefully studied and optimized. The composition of the complex as well as its stability constant was also investigated. The proposed method was applied for the determination of acetazolamide in its tablets and the results obtained were favorably compared with those obtained using the official method. A proposal of the reaction pathway was postulated. Master Publishing Group 2010-06 /pmc/articles/PMC3614737/ /pubmed/23675188 Text en © M. I. Walash et al. Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Walash, M. I. El-Brashy, A. El-Enany, N. Wahba, M. E. K. Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title | Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title_full | Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title_fullStr | Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title_full_unstemmed | Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title_short | Validated Stability indicating Spectrophotometric Method for the Determination of Acetazolamide in Dosage Forms through Complex Formation with Palladium (II) |
title_sort | validated stability indicating spectrophotometric method for the determination of acetazolamide in dosage forms through complex formation with palladium (ii) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614737/ https://www.ncbi.nlm.nih.gov/pubmed/23675188 |
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