A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax

An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary...

Descripción completa

Detalles Bibliográficos
Autores principales: Shrivastava, Sanjay Mohan, Singh, Rajkumar, Tariq, Abu, Siddiqui, Masoom Raza, Yadav, Jitendar, Negi, P. S., Chaudhary, Manu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Master Publishing Group 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614746/
https://www.ncbi.nlm.nih.gov/pubmed/23675112
_version_ 1782264906426351616
author Shrivastava, Sanjay Mohan
Singh, Rajkumar
Tariq, Abu
Siddiqui, Masoom Raza
Yadav, Jitendar
Negi, P. S.
Chaudhary, Manu
author_facet Shrivastava, Sanjay Mohan
Singh, Rajkumar
Tariq, Abu
Siddiqui, Masoom Raza
Yadav, Jitendar
Negi, P. S.
Chaudhary, Manu
author_sort Shrivastava, Sanjay Mohan
collection PubMed
description An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide adjusted to pH7.0 with orthophosphoric acid in ratio 70:30. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 125-750 ppm for ceftriaxone sodium and 62.5-375 ppm for sulbactam sodium. The LC method can be used for the quality control of formulated products containing ceftriaxone and sulbactam.
format Online
Article
Text
id pubmed-3614746
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Master Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-36147462013-05-01 A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax Shrivastava, Sanjay Mohan Singh, Rajkumar Tariq, Abu Siddiqui, Masoom Raza Yadav, Jitendar Negi, P. S. Chaudhary, Manu Int J Biomed Sci Article An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide adjusted to pH7.0 with orthophosphoric acid in ratio 70:30. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 125-750 ppm for ceftriaxone sodium and 62.5-375 ppm for sulbactam sodium. The LC method can be used for the quality control of formulated products containing ceftriaxone and sulbactam. Master Publishing Group 2009-03 /pmc/articles/PMC3614746/ /pubmed/23675112 Text en © S. M. Shrivastava et al. Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Shrivastava, Sanjay Mohan
Singh, Rajkumar
Tariq, Abu
Siddiqui, Masoom Raza
Yadav, Jitendar
Negi, P. S.
Chaudhary, Manu
A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title_full A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title_fullStr A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title_full_unstemmed A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title_short A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax
title_sort novel high performance liquid chromatographic method for simultaneous determination of ceftriaxone and sulbactam in sulbactomax
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614746/
https://www.ncbi.nlm.nih.gov/pubmed/23675112
work_keys_str_mv AT shrivastavasanjaymohan anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT singhrajkumar anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT tariqabu anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT siddiquimasoomraza anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT yadavjitendar anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT negips anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT chaudharymanu anovelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT shrivastavasanjaymohan novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT singhrajkumar novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT tariqabu novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT siddiquimasoomraza novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT yadavjitendar novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT negips novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax
AT chaudharymanu novelhighperformanceliquidchromatographicmethodforsimultaneousdeterminationofceftriaxoneandsulbactaminsulbactomax

Ejemplares similares