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The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment
AIM: Hyperglycemia is known to be associated with an increase in mortality in myocardial infarction and intensive care patients despite the fact that glucose metabolism plays a central role in myocardial protection. We studied the effect of different glucose levels (22 mM L(-1); 5.5 mM L(-1); and 0...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Master Publishing Group
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614748/ https://www.ncbi.nlm.nih.gov/pubmed/23675108 |
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author | Szabó, Zoltán Katkits, Kristofer Gabro, George Andersson, Rolf GG |
author_facet | Szabó, Zoltán Katkits, Kristofer Gabro, George Andersson, Rolf GG |
author_sort | Szabó, Zoltán |
collection | PubMed |
description | AIM: Hyperglycemia is known to be associated with an increase in mortality in myocardial infarction and intensive care patients despite the fact that glucose metabolism plays a central role in myocardial protection. We studied the effect of different glucose levels (22 mM L(-1); 5.5 mM L(-1); and 0 mM L(-1)) on the contractile reserve of isolated rat atrial myocardium during and after hypoxia. METHODS: We observed the contraction of isolated rat atrium strips caused by electrical-field stimulation in a modified Krebs-Henseleit Buffer (KHB) organ bath oxygenated with 95% O(2) + 5% CO(2) at 37°C. We applied two periods of hypoxia and two periods of reoxygenation. Three glucose concentrations were used in the buffer to study the effect of glucose (high- n=6; normal- n=7; and zero-glucose n=6). The effect of isoproterenol 1 μM L(-1) was tested during the second ischemic period. RESULTS: The main finding was that both a zero-glucose (27.8 ± 5.9 vs. 14.7 ± 3 % of baseline tension) and a high-glucose environment (38.5 ± 14 vs. 14.7 ± 3 % of baseline tension) had a positive effect in terms of better contractility than the normal-glucose buffer during both the first (p=0.00062) and the second ischemic period (31.2 ± 5.9 % zero-glucose vs. 14.7 ± 4.2 normal-glucose vs. 35.3 ± 15.9% high-glucose p=0.0038). CONCLUSION: Both zero-glucose and high-glucose environments resulted in a better contractile reserve in isolated rat atrial myocardium during hypoxia than in a normal one. The exact clinical relevance of this observation is, at present, unclear. |
format | Online Article Text |
id | pubmed-3614748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Master Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36147482013-05-01 The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment Szabó, Zoltán Katkits, Kristofer Gabro, George Andersson, Rolf GG Int J Biomed Sci Article AIM: Hyperglycemia is known to be associated with an increase in mortality in myocardial infarction and intensive care patients despite the fact that glucose metabolism plays a central role in myocardial protection. We studied the effect of different glucose levels (22 mM L(-1); 5.5 mM L(-1); and 0 mM L(-1)) on the contractile reserve of isolated rat atrial myocardium during and after hypoxia. METHODS: We observed the contraction of isolated rat atrium strips caused by electrical-field stimulation in a modified Krebs-Henseleit Buffer (KHB) organ bath oxygenated with 95% O(2) + 5% CO(2) at 37°C. We applied two periods of hypoxia and two periods of reoxygenation. Three glucose concentrations were used in the buffer to study the effect of glucose (high- n=6; normal- n=7; and zero-glucose n=6). The effect of isoproterenol 1 μM L(-1) was tested during the second ischemic period. RESULTS: The main finding was that both a zero-glucose (27.8 ± 5.9 vs. 14.7 ± 3 % of baseline tension) and a high-glucose environment (38.5 ± 14 vs. 14.7 ± 3 % of baseline tension) had a positive effect in terms of better contractility than the normal-glucose buffer during both the first (p=0.00062) and the second ischemic period (31.2 ± 5.9 % zero-glucose vs. 14.7 ± 4.2 normal-glucose vs. 35.3 ± 15.9% high-glucose p=0.0038). CONCLUSION: Both zero-glucose and high-glucose environments resulted in a better contractile reserve in isolated rat atrial myocardium during hypoxia than in a normal one. The exact clinical relevance of this observation is, at present, unclear. Master Publishing Group 2009-03 /pmc/articles/PMC3614748/ /pubmed/23675108 Text en © Zoltán Szabó et al. Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Szabó, Zoltán Katkits, Kristofer Gabro, George Andersson, Rolf GG The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title | The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title_full | The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title_fullStr | The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title_full_unstemmed | The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title_short | The Contractility of Isolated Rat Atrial Tissue during Hypoxia is Better Preserved in a High- or Zero-Glucose Environment than in a Normal Glucose Environment |
title_sort | contractility of isolated rat atrial tissue during hypoxia is better preserved in a high- or zero-glucose environment than in a normal glucose environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614748/ https://www.ncbi.nlm.nih.gov/pubmed/23675108 |
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