A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples

A simple and sensitive spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was based on the reaction of drug with the mixture of potassium iodate and potassium iodide. The method was lin...

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Detalles Bibliográficos
Autores principales: Gujral, Rajinder Singh, Haque, Sk Manirul, Shanker, Prem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Master Publishing Group 2009
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614795/
https://www.ncbi.nlm.nih.gov/pubmed/23675167
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author Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
author_facet Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
author_sort Gujral, Rajinder Singh
collection PubMed
description A simple and sensitive spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was based on the reaction of drug with the mixture of potassium iodate and potassium iodide. The method was linear in the range of 0.5–3.5 μg/ml. There is no official method for the determination of pregabalin. The absorbance was measured at 353 nm. The method was validated with respect to accuracy, precision, specificity, ruggedness, robustness, limit of detection and limit of quantitation. This method was used successfully for the quality assessment of five pregabalin drug products and in human urine samples with good precision and accuracy.
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spelling pubmed-36147952013-05-01 A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples Gujral, Rajinder Singh Haque, Sk Manirul Shanker, Prem Int J Biomed Sci Original Article A simple and sensitive spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was based on the reaction of drug with the mixture of potassium iodate and potassium iodide. The method was linear in the range of 0.5–3.5 μg/ml. There is no official method for the determination of pregabalin. The absorbance was measured at 353 nm. The method was validated with respect to accuracy, precision, specificity, ruggedness, robustness, limit of detection and limit of quantitation. This method was used successfully for the quality assessment of five pregabalin drug products and in human urine samples with good precision and accuracy. Master Publishing Group 2009-12 /pmc/articles/PMC3614795/ /pubmed/23675167 Text en © Rajinder Singh Gujral et al. Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title_full A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title_fullStr A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title_full_unstemmed A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title_short A Sensitive Spectrophotometric Method for the Determination of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples
title_sort sensitive spectrophotometric method for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614795/
https://www.ncbi.nlm.nih.gov/pubmed/23675167
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