A Novel Spectrophotometric Method for the Determination Oxacillin Sodium

A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol – dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank. Under...

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Detalles Bibliográficos
Autores principales: Gujral, Rajinder Singh, Haque, Sk Manirul, Shanker, Prem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Master Publishing Group 2009
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614802/
https://www.ncbi.nlm.nih.gov/pubmed/23675161
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author Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
author_facet Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
author_sort Gujral, Rajinder Singh
collection PubMed
description A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol – dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank. Under optimized experimental conditions, Beer’s law is obeyed in the concentration ranges 2–8 μg/ml for Oxacillin Sodium. The method was validated for specificity, linearity, precision, accuracy, limit of quantitation, robustness and ruggedness. The LOD and LOQ value were 0.39 and 1.18 μg/ml respectively. The method was successfully applied to the analysis of Oxacillin sodium in Human Urine samples with good accuracy and precision.
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spelling pubmed-36148022013-05-01 A Novel Spectrophotometric Method for the Determination Oxacillin Sodium Gujral, Rajinder Singh Haque, Sk Manirul Shanker, Prem Int J Biomed Sci Original Article A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol – dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank. Under optimized experimental conditions, Beer’s law is obeyed in the concentration ranges 2–8 μg/ml for Oxacillin Sodium. The method was validated for specificity, linearity, precision, accuracy, limit of quantitation, robustness and ruggedness. The LOD and LOQ value were 0.39 and 1.18 μg/ml respectively. The method was successfully applied to the analysis of Oxacillin sodium in Human Urine samples with good accuracy and precision. Master Publishing Group 2009-12 /pmc/articles/PMC3614802/ /pubmed/23675161 Text en © Rajinder Singh Gujral et al. Licensee Master Publishing Group http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Gujral, Rajinder Singh
Haque, Sk Manirul
Shanker, Prem
A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title_full A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title_fullStr A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title_full_unstemmed A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title_short A Novel Spectrophotometric Method for the Determination Oxacillin Sodium
title_sort novel spectrophotometric method for the determination oxacillin sodium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614802/
https://www.ncbi.nlm.nih.gov/pubmed/23675161
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