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C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and anal...

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Autores principales: Tozaki, Kana, Kimura, Junpei, Yasuda, Masahiro, Ryu, Nobuyuki, Nasu, Testuo, Pernthaner, Anton, Hein, Wayne R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615225/
https://www.ncbi.nlm.nih.gov/pubmed/23388432
http://dx.doi.org/10.4142/jvs.2013.14.1.1
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author Tozaki, Kana
Kimura, Junpei
Yasuda, Masahiro
Ryu, Nobuyuki
Nasu, Testuo
Pernthaner, Anton
Hein, Wayne R.
author_facet Tozaki, Kana
Kimura, Junpei
Yasuda, Masahiro
Ryu, Nobuyuki
Nasu, Testuo
Pernthaner, Anton
Hein, Wayne R.
author_sort Tozaki, Kana
collection PubMed
description The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.
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spelling pubmed-36152252013-04-08 C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches Tozaki, Kana Kimura, Junpei Yasuda, Masahiro Ryu, Nobuyuki Nasu, Testuo Pernthaner, Anton Hein, Wayne R. J Vet Sci Original Article The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens. The Korean Society of Veterinary Science 2013-03 2013-03-24 /pmc/articles/PMC3615225/ /pubmed/23388432 http://dx.doi.org/10.4142/jvs.2013.14.1.1 Text en © 2013 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tozaki, Kana
Kimura, Junpei
Yasuda, Masahiro
Ryu, Nobuyuki
Nasu, Testuo
Pernthaner, Anton
Hein, Wayne R.
C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title_full C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title_fullStr C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title_full_unstemmed C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title_short C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
title_sort c6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal peyer's patches
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615225/
https://www.ncbi.nlm.nih.gov/pubmed/23388432
http://dx.doi.org/10.4142/jvs.2013.14.1.1
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