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Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615227/ https://www.ncbi.nlm.nih.gov/pubmed/23388446 http://dx.doi.org/10.4142/jvs.2013.14.1.15 |
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author | Kang, Jung-Taek Kwon, Dae-Kee Park, Sol-Ji Kim, Su-Jin Moon, Joon-Ho Koo, Ok-Jae Jang, Goo Lee, Byeong-Chun |
author_facet | Kang, Jung-Taek Kwon, Dae-Kee Park, Sol-Ji Kim, Su-Jin Moon, Joon-Ho Koo, Ok-Jae Jang, Goo Lee, Byeong-Chun |
author_sort | Kang, Jung-Taek |
collection | PubMed |
description | Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 µg/mL quercetin during in vitro maturation (IVM). Quercetin treatment did not improve oocyte nuclear maturation, but significantly higher blastocyst rates (p < 0.05) of parthenogenetically activated oocytes were achieved when the IVM medium was supplemented with an adequate concentration of quercetin (1 µg/mL). However, cleavage rates and blastocyst cell numbers were not affected. Oocytes treated with 1 or 10 µg/mL quercetin had significantly lower (p < 0.05) levels of ROS than the control and group treated with the highest concentration of quercetin (50 µg/mL). Moreover, this highest concentration was detrimental to oocyte nuclear maturation and blastocyst formation. Based on our findings, we concluded that exogenous quercetin reduces ROS levels during oocyte maturation and is beneficial for subsequent embryo development. |
format | Online Article Text |
id | pubmed-3615227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36152272013-04-08 Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels Kang, Jung-Taek Kwon, Dae-Kee Park, Sol-Ji Kim, Su-Jin Moon, Joon-Ho Koo, Ok-Jae Jang, Goo Lee, Byeong-Chun J Vet Sci Original Article Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 µg/mL quercetin during in vitro maturation (IVM). Quercetin treatment did not improve oocyte nuclear maturation, but significantly higher blastocyst rates (p < 0.05) of parthenogenetically activated oocytes were achieved when the IVM medium was supplemented with an adequate concentration of quercetin (1 µg/mL). However, cleavage rates and blastocyst cell numbers were not affected. Oocytes treated with 1 or 10 µg/mL quercetin had significantly lower (p < 0.05) levels of ROS than the control and group treated with the highest concentration of quercetin (50 µg/mL). Moreover, this highest concentration was detrimental to oocyte nuclear maturation and blastocyst formation. Based on our findings, we concluded that exogenous quercetin reduces ROS levels during oocyte maturation and is beneficial for subsequent embryo development. The Korean Society of Veterinary Science 2013-03 2013-03-24 /pmc/articles/PMC3615227/ /pubmed/23388446 http://dx.doi.org/10.4142/jvs.2013.14.1.15 Text en © 2013 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kang, Jung-Taek Kwon, Dae-Kee Park, Sol-Ji Kim, Su-Jin Moon, Joon-Ho Koo, Ok-Jae Jang, Goo Lee, Byeong-Chun Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title | Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title_full | Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title_fullStr | Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title_full_unstemmed | Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title_short | Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
title_sort | quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615227/ https://www.ncbi.nlm.nih.gov/pubmed/23388446 http://dx.doi.org/10.4142/jvs.2013.14.1.15 |
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