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Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels

Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the a...

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Autores principales: Kang, Jung-Taek, Kwon, Dae-Kee, Park, Sol-Ji, Kim, Su-Jin, Moon, Joon-Ho, Koo, Ok-Jae, Jang, Goo, Lee, Byeong-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615227/
https://www.ncbi.nlm.nih.gov/pubmed/23388446
http://dx.doi.org/10.4142/jvs.2013.14.1.15
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author Kang, Jung-Taek
Kwon, Dae-Kee
Park, Sol-Ji
Kim, Su-Jin
Moon, Joon-Ho
Koo, Ok-Jae
Jang, Goo
Lee, Byeong-Chun
author_facet Kang, Jung-Taek
Kwon, Dae-Kee
Park, Sol-Ji
Kim, Su-Jin
Moon, Joon-Ho
Koo, Ok-Jae
Jang, Goo
Lee, Byeong-Chun
author_sort Kang, Jung-Taek
collection PubMed
description Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 µg/mL quercetin during in vitro maturation (IVM). Quercetin treatment did not improve oocyte nuclear maturation, but significantly higher blastocyst rates (p < 0.05) of parthenogenetically activated oocytes were achieved when the IVM medium was supplemented with an adequate concentration of quercetin (1 µg/mL). However, cleavage rates and blastocyst cell numbers were not affected. Oocytes treated with 1 or 10 µg/mL quercetin had significantly lower (p < 0.05) levels of ROS than the control and group treated with the highest concentration of quercetin (50 µg/mL). Moreover, this highest concentration was detrimental to oocyte nuclear maturation and blastocyst formation. Based on our findings, we concluded that exogenous quercetin reduces ROS levels during oocyte maturation and is beneficial for subsequent embryo development.
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spelling pubmed-36152272013-04-08 Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels Kang, Jung-Taek Kwon, Dae-Kee Park, Sol-Ji Kim, Su-Jin Moon, Joon-Ho Koo, Ok-Jae Jang, Goo Lee, Byeong-Chun J Vet Sci Original Article Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 µg/mL quercetin during in vitro maturation (IVM). Quercetin treatment did not improve oocyte nuclear maturation, but significantly higher blastocyst rates (p < 0.05) of parthenogenetically activated oocytes were achieved when the IVM medium was supplemented with an adequate concentration of quercetin (1 µg/mL). However, cleavage rates and blastocyst cell numbers were not affected. Oocytes treated with 1 or 10 µg/mL quercetin had significantly lower (p < 0.05) levels of ROS than the control and group treated with the highest concentration of quercetin (50 µg/mL). Moreover, this highest concentration was detrimental to oocyte nuclear maturation and blastocyst formation. Based on our findings, we concluded that exogenous quercetin reduces ROS levels during oocyte maturation and is beneficial for subsequent embryo development. The Korean Society of Veterinary Science 2013-03 2013-03-24 /pmc/articles/PMC3615227/ /pubmed/23388446 http://dx.doi.org/10.4142/jvs.2013.14.1.15 Text en © 2013 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kang, Jung-Taek
Kwon, Dae-Kee
Park, Sol-Ji
Kim, Su-Jin
Moon, Joon-Ho
Koo, Ok-Jae
Jang, Goo
Lee, Byeong-Chun
Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title_full Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title_fullStr Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title_full_unstemmed Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title_short Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
title_sort quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615227/
https://www.ncbi.nlm.nih.gov/pubmed/23388446
http://dx.doi.org/10.4142/jvs.2013.14.1.15
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