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Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)
Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernae...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615513/ https://www.ncbi.nlm.nih.gov/pubmed/23569415 http://dx.doi.org/10.1590/S1415-47572013005000010 |
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author | Rizo, Walace Fraga Ferreira, Luis Eduardo Colnaghi, Vanessa Martins, Juliana Simões Franchi, Leonardo Pereira Takahashi, Catarina Satie Beleboni, Rene Oliveira Marins, Mozart Pereira, Paulo Sérgio Fachin, Ana Lúcia |
author_facet | Rizo, Walace Fraga Ferreira, Luis Eduardo Colnaghi, Vanessa Martins, Juliana Simões Franchi, Leonardo Pereira Takahashi, Catarina Satie Beleboni, Rene Oliveira Marins, Mozart Pereira, Paulo Sérgio Fachin, Ana Lúcia |
author_sort | Rizo, Walace Fraga |
collection | PubMed |
description | Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC(50) = 54.47 μg/mL) than the other alkaloids tested (voacangine IC(50) = 159.33 g/mL, and heyneanine IC(50) = 689.45 μg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit. |
format | Online Article Text |
id | pubmed-3615513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-36155132013-04-08 Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) Rizo, Walace Fraga Ferreira, Luis Eduardo Colnaghi, Vanessa Martins, Juliana Simões Franchi, Leonardo Pereira Takahashi, Catarina Satie Beleboni, Rene Oliveira Marins, Mozart Pereira, Paulo Sérgio Fachin, Ana Lúcia Genet Mol Biol Mutagenesis Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC(50) = 54.47 μg/mL) than the other alkaloids tested (voacangine IC(50) = 159.33 g/mL, and heyneanine IC(50) = 689.45 μg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit. Sociedade Brasileira de Genética 2013-03-04 2013-03 /pmc/articles/PMC3615513/ /pubmed/23569415 http://dx.doi.org/10.1590/S1415-47572013005000010 Text en Copyright © 2013, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Mutagenesis Rizo, Walace Fraga Ferreira, Luis Eduardo Colnaghi, Vanessa Martins, Juliana Simões Franchi, Leonardo Pereira Takahashi, Catarina Satie Beleboni, Rene Oliveira Marins, Mozart Pereira, Paulo Sérgio Fachin, Ana Lúcia Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title | Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title_full | Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title_fullStr | Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title_full_unstemmed | Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title_short | Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) |
title_sort | cytotoxicity and genotoxicity of coronaridine from tabernaemontana catharinensis a.dc in a human laryngeal epithelial carcinoma cell line (hep-2) |
topic | Mutagenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615513/ https://www.ncbi.nlm.nih.gov/pubmed/23569415 http://dx.doi.org/10.1590/S1415-47572013005000010 |
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