How to measure RNA expression in rare senescent cells expressing any specific protein such as p16(Ink4a)

Here we describe a carefully optimized method for the preparation of high quality RNA by flow sorting of formaldehyde fixed senescent cells immunostained for any intracellular antigen. Replicative cellular senescence is a phenomenon of irreversible growth arrest triggered by the accumulation of a di...

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Detalles Bibliográficos
Autores principales: Jeyapalan, Jessie C., Sedivy, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616199/
https://www.ncbi.nlm.nih.gov/pubmed/23454889

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