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Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats
The aim of this study was to investigate food intake, serum leptin levels, and leptin mRNA expression during the sexual cycle in rats. Female Wistar-Imamichi rats aged 8-10 weeks were used in this experiment. Food intake was measured during the light and dark phases (light on at 07:00 and off at 19:...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association for Laboratory Animal Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616203/ https://www.ncbi.nlm.nih.gov/pubmed/23573101 http://dx.doi.org/10.5625/lar.2013.29.1.1 |
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author | Fungfuang, Wirasak Nakada, Tomoaki Nakao, Nobuhiro Terada, Misao Yokosuka, Makoto Gizurarson, Sveinbjorn Hau, Jann Moon, Changjong Saito, Toru R. |
author_facet | Fungfuang, Wirasak Nakada, Tomoaki Nakao, Nobuhiro Terada, Misao Yokosuka, Makoto Gizurarson, Sveinbjorn Hau, Jann Moon, Changjong Saito, Toru R. |
author_sort | Fungfuang, Wirasak |
collection | PubMed |
description | The aim of this study was to investigate food intake, serum leptin levels, and leptin mRNA expression during the sexual cycle in rats. Female Wistar-Imamichi rats aged 8-10 weeks were used in this experiment. Food intake was measured during the light and dark phases (light on at 07:00 and off at 19:00) of the 4-day estrous cycle in female rats. Serum leptin levels were measured by ELISA, and leptin mRNA expression levels were analyzed using real-time PCR on diestrous- and proestrous-stage rats. Our results revealed that during the sexual cycle, food intake was significantly higher in the dark phase compared with the light phase. Food intake in proestrous females was significantly lower in the light and dark phases compared with the other groups. Serum leptin concentrations were significantly higher in both phases in proestrous rats compared with diestrous rats. There was a significant increase in leptin mRNA expression in adipose tissue during the proestrous period compared with the diestrous period. These findings suggest that increased leptin mRNA expression and serum leptin levels, which are induced by estrogen during the proestrous stage, may play a role in regulating appetitive behavior. |
format | Online Article Text |
id | pubmed-3616203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Korean Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36162032013-04-09 Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats Fungfuang, Wirasak Nakada, Tomoaki Nakao, Nobuhiro Terada, Misao Yokosuka, Makoto Gizurarson, Sveinbjorn Hau, Jann Moon, Changjong Saito, Toru R. Lab Anim Res Original Article The aim of this study was to investigate food intake, serum leptin levels, and leptin mRNA expression during the sexual cycle in rats. Female Wistar-Imamichi rats aged 8-10 weeks were used in this experiment. Food intake was measured during the light and dark phases (light on at 07:00 and off at 19:00) of the 4-day estrous cycle in female rats. Serum leptin levels were measured by ELISA, and leptin mRNA expression levels were analyzed using real-time PCR on diestrous- and proestrous-stage rats. Our results revealed that during the sexual cycle, food intake was significantly higher in the dark phase compared with the light phase. Food intake in proestrous females was significantly lower in the light and dark phases compared with the other groups. Serum leptin concentrations were significantly higher in both phases in proestrous rats compared with diestrous rats. There was a significant increase in leptin mRNA expression in adipose tissue during the proestrous period compared with the diestrous period. These findings suggest that increased leptin mRNA expression and serum leptin levels, which are induced by estrogen during the proestrous stage, may play a role in regulating appetitive behavior. Korean Association for Laboratory Animal Science 2013-03 2013-03-25 /pmc/articles/PMC3616203/ /pubmed/23573101 http://dx.doi.org/10.5625/lar.2013.29.1.1 Text en Copyright © 2013 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Fungfuang, Wirasak Nakada, Tomoaki Nakao, Nobuhiro Terada, Misao Yokosuka, Makoto Gizurarson, Sveinbjorn Hau, Jann Moon, Changjong Saito, Toru R. Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title | Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title_full | Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title_fullStr | Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title_full_unstemmed | Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title_short | Serum leptin concentrations, leptin mRNA expression, and food intake during the estrous cycle in rats |
title_sort | serum leptin concentrations, leptin mrna expression, and food intake during the estrous cycle in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616203/ https://www.ncbi.nlm.nih.gov/pubmed/23573101 http://dx.doi.org/10.5625/lar.2013.29.1.1 |
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