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Stereological study of the diabetic heart of male rats

The present study aimed to quantitatively compare the normal and diabetic hearts of rats using stereological methods. Diabetic and control rats received streptozotocin (60 mg/kg) and no treatments, respectively. On the 56(th) day, the hearts were removed and their total volume was estimated using is...

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Autores principales: Noorafshan, Ali, Khazraei, Hajar, Mirkhani, Hossein, Karbalay-Doust, Saied
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association for Laboratory Animal Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616204/
https://www.ncbi.nlm.nih.gov/pubmed/23573103
http://dx.doi.org/10.5625/lar.2013.29.1.12
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author Noorafshan, Ali
Khazraei, Hajar
Mirkhani, Hossein
Karbalay-Doust, Saied
author_facet Noorafshan, Ali
Khazraei, Hajar
Mirkhani, Hossein
Karbalay-Doust, Saied
author_sort Noorafshan, Ali
collection PubMed
description The present study aimed to quantitatively compare the normal and diabetic hearts of rats using stereological methods. Diabetic and control rats received streptozotocin (60 mg/kg) and no treatments, respectively. On the 56(th) day, the hearts were removed and their total volume was estimated using isotropic Cavalieri method. The total volume of the connective tissues and vessels, total length and diameter of the vessels, total number of cardiomyocytes nuclei, and the mean volume of the cardiomyocytes were estimated, as well. In comparison to the control animals, 60 and 43% increase was observed in the total volume of the connective tissue and microvessels of the diabetic rats, respectively (P<0.05). The percent of the vessel profiles with the diameter of 2-4 µm was decreased, while the percent of the vessel profiles with the diameter of 4.1-8 µm was increased in the diabetic hearts (P<0.05). No significant difference was found in the vessels with more than 8 µm diameters. The total number of the cardiomyocytes' nuclei and the number-weighted mean volume were respectively decreased by 37 and 64% in the diabetic group (P<0.01). A significant difference was observed between the two groups concerning the left ventricle volume to body weight ratio as an index for ventricular hypertrophy (P<0.05), while no difference was found regarding the right ventricle to body weight ratio. It can be concluded that diabetes can induce structural changes, including loss and/or atrophy of the cardiomyocytes, accompanied with increase in the connective tissue in the rats' hearts.
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spelling pubmed-36162042013-04-09 Stereological study of the diabetic heart of male rats Noorafshan, Ali Khazraei, Hajar Mirkhani, Hossein Karbalay-Doust, Saied Lab Anim Res Original Article The present study aimed to quantitatively compare the normal and diabetic hearts of rats using stereological methods. Diabetic and control rats received streptozotocin (60 mg/kg) and no treatments, respectively. On the 56(th) day, the hearts were removed and their total volume was estimated using isotropic Cavalieri method. The total volume of the connective tissues and vessels, total length and diameter of the vessels, total number of cardiomyocytes nuclei, and the mean volume of the cardiomyocytes were estimated, as well. In comparison to the control animals, 60 and 43% increase was observed in the total volume of the connective tissue and microvessels of the diabetic rats, respectively (P<0.05). The percent of the vessel profiles with the diameter of 2-4 µm was decreased, while the percent of the vessel profiles with the diameter of 4.1-8 µm was increased in the diabetic hearts (P<0.05). No significant difference was found in the vessels with more than 8 µm diameters. The total number of the cardiomyocytes' nuclei and the number-weighted mean volume were respectively decreased by 37 and 64% in the diabetic group (P<0.01). A significant difference was observed between the two groups concerning the left ventricle volume to body weight ratio as an index for ventricular hypertrophy (P<0.05), while no difference was found regarding the right ventricle to body weight ratio. It can be concluded that diabetes can induce structural changes, including loss and/or atrophy of the cardiomyocytes, accompanied with increase in the connective tissue in the rats' hearts. Korean Association for Laboratory Animal Science 2013-03 2013-03-25 /pmc/articles/PMC3616204/ /pubmed/23573103 http://dx.doi.org/10.5625/lar.2013.29.1.12 Text en Copyright © 2013 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Noorafshan, Ali
Khazraei, Hajar
Mirkhani, Hossein
Karbalay-Doust, Saied
Stereological study of the diabetic heart of male rats
title Stereological study of the diabetic heart of male rats
title_full Stereological study of the diabetic heart of male rats
title_fullStr Stereological study of the diabetic heart of male rats
title_full_unstemmed Stereological study of the diabetic heart of male rats
title_short Stereological study of the diabetic heart of male rats
title_sort stereological study of the diabetic heart of male rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616204/
https://www.ncbi.nlm.nih.gov/pubmed/23573103
http://dx.doi.org/10.5625/lar.2013.29.1.12
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