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Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle

BACKGROUND: Toxoplasmosis is caused by the apicomplexan parasite Toxoplasma gondii and can be acquired either congenitally or via the oral route. In the latter case, transmission is mediated by two distinct invasive stages, i.e., bradyzoites residing in tissue cysts or sporozoites contained in envir...

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Autores principales: Possenti, Alessia, Fratini, Federica, Fantozzi, Luca, Pozio, Edoardo, Dubey, Jitender P, Ponzi, Marta, Pizzi, Elisabetta, Spano, Furio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616887/
https://www.ncbi.nlm.nih.gov/pubmed/23496850
http://dx.doi.org/10.1186/1471-2164-14-183
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author Possenti, Alessia
Fratini, Federica
Fantozzi, Luca
Pozio, Edoardo
Dubey, Jitender P
Ponzi, Marta
Pizzi, Elisabetta
Spano, Furio
author_facet Possenti, Alessia
Fratini, Federica
Fantozzi, Luca
Pozio, Edoardo
Dubey, Jitender P
Ponzi, Marta
Pizzi, Elisabetta
Spano, Furio
author_sort Possenti, Alessia
collection PubMed
description BACKGROUND: Toxoplasmosis is caused by the apicomplexan parasite Toxoplasma gondii and can be acquired either congenitally or via the oral route. In the latter case, transmission is mediated by two distinct invasive stages, i.e., bradyzoites residing in tissue cysts or sporozoites contained in environmentally resistant oocysts shed by felids in their feces. The oocyst plays a central epidemiological role, yet this stage has been scarcely investigated at the molecular level and the knowledge of its expressed proteome is very limited. RESULTS: Using one-dimensional gel electrophoresis coupled to liquid chromatography-linked tandem mass spectrometry, we analysed total or fractionated protein extracts of partially sporulated T. gondii oocysts, producing a dataset of 1304 non reduntant proteins (~18% of the total predicted proteome), ~59% of which were classified according to the MIPS functional catalogue database. Notably, the comparison of the oocyst dataset with the extensively covered proteome of T. gondii tachyzoite, the invasive stage responsible for the clinical signs of toxoplasmosis, identified 154 putative oocyst/sporozoite-specific proteins, some of which were validated by Western blot. The analysis of this protein subset showed that, compared to tachyzoites, oocysts have a greater capability of de novo amino acid biosynthesis and are well equipped to fuel the Krebs cycle with the acetyl-CoA generated through fatty acid β-oxidation and the degradation of branched amino acids. CONCLUSIONS: The study reported herein significantly expanded our knowledge of the proteome expressed by the oocyst/sporozoite of T. gondii, shedding light on a stage-specifc subset of proteins whose functional profile is consistent with the adaptation of T. gondii oocysts to the nutrient-poor and stressing extracellular environment.
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spelling pubmed-36168872013-04-05 Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle Possenti, Alessia Fratini, Federica Fantozzi, Luca Pozio, Edoardo Dubey, Jitender P Ponzi, Marta Pizzi, Elisabetta Spano, Furio BMC Genomics Research Article BACKGROUND: Toxoplasmosis is caused by the apicomplexan parasite Toxoplasma gondii and can be acquired either congenitally or via the oral route. In the latter case, transmission is mediated by two distinct invasive stages, i.e., bradyzoites residing in tissue cysts or sporozoites contained in environmentally resistant oocysts shed by felids in their feces. The oocyst plays a central epidemiological role, yet this stage has been scarcely investigated at the molecular level and the knowledge of its expressed proteome is very limited. RESULTS: Using one-dimensional gel electrophoresis coupled to liquid chromatography-linked tandem mass spectrometry, we analysed total or fractionated protein extracts of partially sporulated T. gondii oocysts, producing a dataset of 1304 non reduntant proteins (~18% of the total predicted proteome), ~59% of which were classified according to the MIPS functional catalogue database. Notably, the comparison of the oocyst dataset with the extensively covered proteome of T. gondii tachyzoite, the invasive stage responsible for the clinical signs of toxoplasmosis, identified 154 putative oocyst/sporozoite-specific proteins, some of which were validated by Western blot. The analysis of this protein subset showed that, compared to tachyzoites, oocysts have a greater capability of de novo amino acid biosynthesis and are well equipped to fuel the Krebs cycle with the acetyl-CoA generated through fatty acid β-oxidation and the degradation of branched amino acids. CONCLUSIONS: The study reported herein significantly expanded our knowledge of the proteome expressed by the oocyst/sporozoite of T. gondii, shedding light on a stage-specifc subset of proteins whose functional profile is consistent with the adaptation of T. gondii oocysts to the nutrient-poor and stressing extracellular environment. BioMed Central 2013-03-15 /pmc/articles/PMC3616887/ /pubmed/23496850 http://dx.doi.org/10.1186/1471-2164-14-183 Text en Copyright © 2013 Possenti et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Possenti, Alessia
Fratini, Federica
Fantozzi, Luca
Pozio, Edoardo
Dubey, Jitender P
Ponzi, Marta
Pizzi, Elisabetta
Spano, Furio
Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title_full Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title_fullStr Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title_full_unstemmed Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title_short Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
title_sort global proteomic analysis of the oocyst/sporozoite of toxoplasma gondii reveals commitment to a host-independent lifestyle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616887/
https://www.ncbi.nlm.nih.gov/pubmed/23496850
http://dx.doi.org/10.1186/1471-2164-14-183
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