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Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells

In this paper, we report a light driven, non-invasive cell membrane perforation technique based on the localized field amplification by a nanosecond pulsed laser near gold nanoparticles (AuNPs). The optoporation phenomena is investigated with pulses generated by a Nd:YAG laser for two wavelengths th...

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Autores principales: St-Louis Lalonde, Bastien, Boulais, Étienne, Lebrun, Jean-Jacques, Meunier, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617711/
https://www.ncbi.nlm.nih.gov/pubmed/23577284
http://dx.doi.org/10.1364/BOE.4.000490
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author St-Louis Lalonde, Bastien
Boulais, Étienne
Lebrun, Jean-Jacques
Meunier, Michel
author_facet St-Louis Lalonde, Bastien
Boulais, Étienne
Lebrun, Jean-Jacques
Meunier, Michel
author_sort St-Louis Lalonde, Bastien
collection PubMed
description In this paper, we report a light driven, non-invasive cell membrane perforation technique based on the localized field amplification by a nanosecond pulsed laser near gold nanoparticles (AuNPs). The optoporation phenomena is investigated with pulses generated by a Nd:YAG laser for two wavelengths that are either in the visible (532 nm) or near infrared (NIR) (1064 nm). Here, the main objective is to compare on and off localized surface plasmonic resonance (LSPR) to introduce foreign material through the cell membrane using nanosecond laser pulses. The membrane permeability of human melanoma cells (MW278) has been successfully increased as shown by the intake of a fluorescent dye upon irradiation. The viability of this laser driven perforation method is evaluated by propidium iodide exclusion as well as MTT assay. Our results show that up to 25% of the cells are perforated with 532 nm pulses at 50 mJ/cm(2) and around 30% of the cells are perforated with 1064 nm pulses at 1 J/cm(2). With 532 nm pulses, the viability 2 h after treatment is 64% but it increases to 88% 72 h later. On the other hand, the irradiation with 1064 nm pulses leads to an improved 2 h viability of 81% and reaches 98% after 72 h. Scanning electron microscopy images show that the 5 pulses delivered during treatment induce changes in the AuNPs size distribution when irradiated by a 532 nm beam, while this distribution is barely affected when 1064 nm is used.
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spelling pubmed-36177112013-04-10 Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells St-Louis Lalonde, Bastien Boulais, Étienne Lebrun, Jean-Jacques Meunier, Michel Biomed Opt Express Cell Studies In this paper, we report a light driven, non-invasive cell membrane perforation technique based on the localized field amplification by a nanosecond pulsed laser near gold nanoparticles (AuNPs). The optoporation phenomena is investigated with pulses generated by a Nd:YAG laser for two wavelengths that are either in the visible (532 nm) or near infrared (NIR) (1064 nm). Here, the main objective is to compare on and off localized surface plasmonic resonance (LSPR) to introduce foreign material through the cell membrane using nanosecond laser pulses. The membrane permeability of human melanoma cells (MW278) has been successfully increased as shown by the intake of a fluorescent dye upon irradiation. The viability of this laser driven perforation method is evaluated by propidium iodide exclusion as well as MTT assay. Our results show that up to 25% of the cells are perforated with 532 nm pulses at 50 mJ/cm(2) and around 30% of the cells are perforated with 1064 nm pulses at 1 J/cm(2). With 532 nm pulses, the viability 2 h after treatment is 64% but it increases to 88% 72 h later. On the other hand, the irradiation with 1064 nm pulses leads to an improved 2 h viability of 81% and reaches 98% after 72 h. Scanning electron microscopy images show that the 5 pulses delivered during treatment induce changes in the AuNPs size distribution when irradiated by a 532 nm beam, while this distribution is barely affected when 1064 nm is used. Optical Society of America 2013-03-01 /pmc/articles/PMC3617711/ /pubmed/23577284 http://dx.doi.org/10.1364/BOE.4.000490 Text en ©2013 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Cell Studies
St-Louis Lalonde, Bastien
Boulais, Étienne
Lebrun, Jean-Jacques
Meunier, Michel
Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title_full Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title_fullStr Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title_full_unstemmed Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title_short Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
title_sort visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
topic Cell Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617711/
https://www.ncbi.nlm.nih.gov/pubmed/23577284
http://dx.doi.org/10.1364/BOE.4.000490
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