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Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa
The nucleoskeleton of plants contains a peripheral lamina (also called plamina) and, even though lamins are absent in plants, their roles are still fulfilled in plant nuclei. One of the most intriguing topics in plant biology concerns the identity of lamin protein analogues in plants. Good candidate...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617829/ https://www.ncbi.nlm.nih.gov/pubmed/23378381 http://dx.doi.org/10.1093/jxb/ert020 |
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author | Ciska, Malgorzata Masuda, Kiyoshi Moreno Díaz de la Espina, Susana |
author_facet | Ciska, Malgorzata Masuda, Kiyoshi Moreno Díaz de la Espina, Susana |
author_sort | Ciska, Malgorzata |
collection | PubMed |
description | The nucleoskeleton of plants contains a peripheral lamina (also called plamina) and, even though lamins are absent in plants, their roles are still fulfilled in plant nuclei. One of the most intriguing topics in plant biology concerns the identity of lamin protein analogues in plants. Good candidates to play lamin functions in plants are the members of the NMCP (nuclear matrix constituent protein) family, which exhibit the typical tripartite structure of lamins. This paper describes a bioinformatics analysis and classification of the NMCP family based on phylogenetic relationships, sequence similarity and the distribution of conserved regions in 76 homologues. In addition, NMCP1 in the monocot Allium cepa characterized by its sequence and structure, biochemical properties, and subnuclear distribution and alterations in its expression throughout the root were identified. The results demonstrate that these proteins exhibit many similarities to lamins (structural organization, conserved regions, subnuclear distribution, and solubility) and that they may fulfil the functions of lamins in plants. These findings significantly advance understanding of the structural proteins of the plant lamina and nucleoskeleton and provide a basis for further investigation of the protein networks forming these structures. |
format | Online Article Text |
id | pubmed-3617829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36178292013-04-08 Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa Ciska, Malgorzata Masuda, Kiyoshi Moreno Díaz de la Espina, Susana J Exp Bot Research Paper The nucleoskeleton of plants contains a peripheral lamina (also called plamina) and, even though lamins are absent in plants, their roles are still fulfilled in plant nuclei. One of the most intriguing topics in plant biology concerns the identity of lamin protein analogues in plants. Good candidates to play lamin functions in plants are the members of the NMCP (nuclear matrix constituent protein) family, which exhibit the typical tripartite structure of lamins. This paper describes a bioinformatics analysis and classification of the NMCP family based on phylogenetic relationships, sequence similarity and the distribution of conserved regions in 76 homologues. In addition, NMCP1 in the monocot Allium cepa characterized by its sequence and structure, biochemical properties, and subnuclear distribution and alterations in its expression throughout the root were identified. The results demonstrate that these proteins exhibit many similarities to lamins (structural organization, conserved regions, subnuclear distribution, and solubility) and that they may fulfil the functions of lamins in plants. These findings significantly advance understanding of the structural proteins of the plant lamina and nucleoskeleton and provide a basis for further investigation of the protein networks forming these structures. Oxford University Press 2013-04 2013-02-01 /pmc/articles/PMC3617829/ /pubmed/23378381 http://dx.doi.org/10.1093/jxb/ert020 Text en © The Author(2) [2013]. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. |
spellingShingle | Research Paper Ciska, Malgorzata Masuda, Kiyoshi Moreno Díaz de la Espina, Susana Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title | Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title_full | Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title_fullStr | Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title_full_unstemmed | Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title_short | Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa |
title_sort | lamin-like analogues in plants: the characterization of nmcp1 in allium cepa |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617829/ https://www.ncbi.nlm.nih.gov/pubmed/23378381 http://dx.doi.org/10.1093/jxb/ert020 |
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