Cargando…

Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis

Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LT...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Lin, Yang, Haibian, Zhang, Xiaoyan, Yang, Shuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617838/
https://www.ncbi.nlm.nih.gov/pubmed/23404903
http://dx.doi.org/10.1093/jxb/ert040
_version_ 1782265318323781632
author Guo, Lin
Yang, Haibian
Zhang, Xiaoyan
Yang, Shuhua
author_facet Guo, Lin
Yang, Haibian
Zhang, Xiaoyan
Yang, Shuhua
author_sort Guo, Lin
collection PubMed
description Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress.
format Online
Article
Text
id pubmed-3617838
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-36178382013-04-08 Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis Guo, Lin Yang, Haibian Zhang, Xiaoyan Yang, Shuhua J Exp Bot Research Paper Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress. Oxford University Press 2013-04 2013-02-11 /pmc/articles/PMC3617838/ /pubmed/23404903 http://dx.doi.org/10.1093/jxb/ert040 Text en © The Author(2) [2013]. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Research Paper
Guo, Lin
Yang, Haibian
Zhang, Xiaoyan
Yang, Shuhua
Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title_full Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title_fullStr Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title_full_unstemmed Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title_short Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
title_sort lipid transfer protein 3 as a target of myb96 mediates freezing and drought stress in arabidopsis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617838/
https://www.ncbi.nlm.nih.gov/pubmed/23404903
http://dx.doi.org/10.1093/jxb/ert040
work_keys_str_mv AT guolin lipidtransferprotein3asatargetofmyb96mediatesfreezinganddroughtstressinarabidopsis
AT yanghaibian lipidtransferprotein3asatargetofmyb96mediatesfreezinganddroughtstressinarabidopsis
AT zhangxiaoyan lipidtransferprotein3asatargetofmyb96mediatesfreezinganddroughtstressinarabidopsis
AT yangshuhua lipidtransferprotein3asatargetofmyb96mediatesfreezinganddroughtstressinarabidopsis