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An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce

We have generated an ultra-high-density genetic map for lettuce, an economically important member of the Compositae, consisting of 12,842 unigenes (13,943 markers) mapped in 3696 genetic bins distributed over nine chromosomal linkage groups. Genomic DNA was hybridized to a custom Affymetrix oligonuc...

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Autores principales: Truco, Maria José, Ashrafi, Hamid, Kozik, Alexander, van Leeuwen, Hans, Bowers, John, Wo, Sebastian Reyes Chin, Stoffel, Kevin, Xu, Huaqin, Hill, Theresa, Van Deynze, Allen, Michelmore, Richard W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618349/
https://www.ncbi.nlm.nih.gov/pubmed/23550116
http://dx.doi.org/10.1534/g3.112.004929
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author Truco, Maria José
Ashrafi, Hamid
Kozik, Alexander
van Leeuwen, Hans
Bowers, John
Wo, Sebastian Reyes Chin
Stoffel, Kevin
Xu, Huaqin
Hill, Theresa
Van Deynze, Allen
Michelmore, Richard W.
author_facet Truco, Maria José
Ashrafi, Hamid
Kozik, Alexander
van Leeuwen, Hans
Bowers, John
Wo, Sebastian Reyes Chin
Stoffel, Kevin
Xu, Huaqin
Hill, Theresa
Van Deynze, Allen
Michelmore, Richard W.
author_sort Truco, Maria José
collection PubMed
description We have generated an ultra-high-density genetic map for lettuce, an economically important member of the Compositae, consisting of 12,842 unigenes (13,943 markers) mapped in 3696 genetic bins distributed over nine chromosomal linkage groups. Genomic DNA was hybridized to a custom Affymetrix oligonucleotide array containing 6.4 million features representing 35,628 unigenes of Lactuca spp. Segregation of single-position polymorphisms was analyzed using 213 F(7:8) recombinant inbred lines that had been generated by crossing cultivated Lactuca sativa cv. Salinas and L. serriola acc. US96UC23, the wild progenitor species of L. sativa. The high level of replication of each allele in the recombinant inbred lines was exploited to identify single-position polymorphisms that were assigned to parental haplotypes. Marker information has been made available using GBrowse to facilitate access to the map. This map has been anchored to the previously published integrated map of lettuce providing candidate genes for multiple phenotypes. The high density of markers achieved in this ultradense map allowed syntenic studies between lettuce and Vitis vinifera as well as other plant species.
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spelling pubmed-36183492013-04-08 An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce Truco, Maria José Ashrafi, Hamid Kozik, Alexander van Leeuwen, Hans Bowers, John Wo, Sebastian Reyes Chin Stoffel, Kevin Xu, Huaqin Hill, Theresa Van Deynze, Allen Michelmore, Richard W. G3 (Bethesda) Investigations We have generated an ultra-high-density genetic map for lettuce, an economically important member of the Compositae, consisting of 12,842 unigenes (13,943 markers) mapped in 3696 genetic bins distributed over nine chromosomal linkage groups. Genomic DNA was hybridized to a custom Affymetrix oligonucleotide array containing 6.4 million features representing 35,628 unigenes of Lactuca spp. Segregation of single-position polymorphisms was analyzed using 213 F(7:8) recombinant inbred lines that had been generated by crossing cultivated Lactuca sativa cv. Salinas and L. serriola acc. US96UC23, the wild progenitor species of L. sativa. The high level of replication of each allele in the recombinant inbred lines was exploited to identify single-position polymorphisms that were assigned to parental haplotypes. Marker information has been made available using GBrowse to facilitate access to the map. This map has been anchored to the previously published integrated map of lettuce providing candidate genes for multiple phenotypes. The high density of markers achieved in this ultradense map allowed syntenic studies between lettuce and Vitis vinifera as well as other plant species. Genetics Society of America 2013-04-01 /pmc/articles/PMC3618349/ /pubmed/23550116 http://dx.doi.org/10.1534/g3.112.004929 Text en Copyright © 2013 Truco et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Truco, Maria José
Ashrafi, Hamid
Kozik, Alexander
van Leeuwen, Hans
Bowers, John
Wo, Sebastian Reyes Chin
Stoffel, Kevin
Xu, Huaqin
Hill, Theresa
Van Deynze, Allen
Michelmore, Richard W.
An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title_full An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title_fullStr An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title_full_unstemmed An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title_short An Ultra-High-Density, Transcript-Based, Genetic Map of Lettuce
title_sort ultra-high-density, transcript-based, genetic map of lettuce
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618349/
https://www.ncbi.nlm.nih.gov/pubmed/23550116
http://dx.doi.org/10.1534/g3.112.004929
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