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Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis
BACKGROUND: The membrane mucin MUC1 is altered in its pattern of expression in cancer, and also in other pathological situations, including Helicobacter pylori gastritis. Here we investigate the basis for the loss of apical staining of the gastric foveolar epithelium in H. pylori gastritis. METHODS:...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619071/ https://www.ncbi.nlm.nih.gov/pubmed/23412104 http://dx.doi.org/10.1038/bjc.2013.54 |
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author | Rashid, M Teixeira, A S Qureshi, U Pereira, S P Novelli, M R Swallow, D M |
author_facet | Rashid, M Teixeira, A S Qureshi, U Pereira, S P Novelli, M R Swallow, D M |
author_sort | Rashid, M |
collection | PubMed |
description | BACKGROUND: The membrane mucin MUC1 is altered in its pattern of expression in cancer, and also in other pathological situations, including Helicobacter pylori gastritis. Here we investigate the basis for the loss of apical staining of the gastric foveolar epithelium in H. pylori gastritis. METHODS: MUC1 was examined in the gastric antrum from cases of H. pylori gastritis and normal controls. We used tissue sections that were either treated or not treated with periodate to effect deglycosylation, and the monoclonal antibodies LICRLonM8, MUSE-11, CT2 and BC2. RESULTS: We show that the epitopes on the TR domain of MUC1 are partially cryptic due to glycosylation and that MUC1 is present on the apical surface of the gastric foveolar epithelium of gastritis patients. CONCLUSION: This observation suggests that there is no substantial loss of the mucin domain of MUC1 from the apical surface in gastritis, as suggested by others, but rather the H. pylori influences the glycosylation of MUC1. This paper highlights the issue of epitope specificity of monoclonal antibodies directed against disease-associated markers, specifically when they are glycoproteins, as is the case for many cancer markers. |
format | Online Article Text |
id | pubmed-3619071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36190712014-03-19 Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis Rashid, M Teixeira, A S Qureshi, U Pereira, S P Novelli, M R Swallow, D M Br J Cancer Molecular Diagnostics BACKGROUND: The membrane mucin MUC1 is altered in its pattern of expression in cancer, and also in other pathological situations, including Helicobacter pylori gastritis. Here we investigate the basis for the loss of apical staining of the gastric foveolar epithelium in H. pylori gastritis. METHODS: MUC1 was examined in the gastric antrum from cases of H. pylori gastritis and normal controls. We used tissue sections that were either treated or not treated with periodate to effect deglycosylation, and the monoclonal antibodies LICRLonM8, MUSE-11, CT2 and BC2. RESULTS: We show that the epitopes on the TR domain of MUC1 are partially cryptic due to glycosylation and that MUC1 is present on the apical surface of the gastric foveolar epithelium of gastritis patients. CONCLUSION: This observation suggests that there is no substantial loss of the mucin domain of MUC1 from the apical surface in gastritis, as suggested by others, but rather the H. pylori influences the glycosylation of MUC1. This paper highlights the issue of epitope specificity of monoclonal antibodies directed against disease-associated markers, specifically when they are glycoproteins, as is the case for many cancer markers. Nature Publishing Group 2013-03-19 2013-02-14 /pmc/articles/PMC3619071/ /pubmed/23412104 http://dx.doi.org/10.1038/bjc.2013.54 Text en Copyright © 2013 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Molecular Diagnostics Rashid, M Teixeira, A S Qureshi, U Pereira, S P Novelli, M R Swallow, D M Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title | Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title_full | Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title_fullStr | Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title_full_unstemmed | Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title_short | Apical MUC1 expression revealed on the foveolar epithelium in H. pylori gastritis |
title_sort | apical muc1 expression revealed on the foveolar epithelium in h. pylori gastritis |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3619071/ https://www.ncbi.nlm.nih.gov/pubmed/23412104 http://dx.doi.org/10.1038/bjc.2013.54 |
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